Linked Life Data

12481100

Source:http://linkedlifedata.com/resource/pubmed/id/12481100

Statements in which the resource exists.
SubjectPredicateObjectContext
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pubmed-article:12481100pubmed:dateCreated2002-12-13lld:pubmed
pubmed-article:12481100pubmed:abstractTextA beta-primeverosidase from tea (Camellia sinensis) plants is a unique disaccharide-specific glycosidase, which hydrolyzes aroma precursors of beta-primeverosides (6-O-beta-D-xylopyranosyl-beta-D-glucopyranosides) to liberate various aroma compounds, and the enzyme is deeply concerned with the floral aroma formation in oolong tea and black tea during the manufacturing process. The beta-primeverosidase was purified from fresh leaves of a cultivar for green tea (C. sinensis var sinensis cv Yabukita), and its partial amino acid sequences were determined. The beta-primeverosidase cDNA has been isolated from a cDNA library of cv Yabukita using degenerate oligonucleotide primers. The cDNA insert encodes a polypeptide consisting of an N-terminal signal peptide of 28 amino acid residues and a 479-amino acid mature protein. The beta-primeverosidase protein sequence was 50% to 60% identical to beta-glucosidases from various plants and was classified in a family 1 glycosyl hydrolase. The mature form of the beta-primeverosidase expressed in Escherichia coli was able to hydrolyze beta-primeverosides to liberate a primeverose unit and aglycons, but did not act on 2-phenylethyl beta-D-glucopyranoside. These results indicate that the beta-primeverosidase selectively recognizes the beta-primeverosides as substrates and specifically hydrolyzes the beta-glycosidic bond between the disaccharide and the aglycons. The stereochemistry for enzymatic hydrolysis of 2-phenylethyl beta-primeveroside by the beta-primeverosidase was followed by (1)H-nuclear magnetic resonance spectroscopy, revealing that the enzyme hydrolyzes the beta-primeveroside by a retaining mechanism. The roles of the beta-primeverosidase in the defense mechanism in tea plants and the floral aroma formation during tea manufacturing process are also discussed.lld:pubmed
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pubmed-article:12481100pubmed:authorpubmed-author:NakaoMasahiro...lld:pubmed
pubmed-article:12481100pubmed:authorpubmed-author:MaSeung-JinSJlld:pubmed
pubmed-article:12481100pubmed:authorpubmed-author:SakataKanzoKlld:pubmed
pubmed-article:12481100pubmed:authorpubmed-author:Fukuchi-Mizut...lld:pubmed
pubmed-article:12481100pubmed:authorpubmed-author:MizutaniMasah...lld:pubmed
pubmed-article:12481100pubmed:authorpubmed-author:NakanishiHide...lld:pubmed
pubmed-article:12481100pubmed:authorpubmed-author:EmaJun-ichiJlld:pubmed
pubmed-article:12481100pubmed:authorpubmed-author:NoguchiEtsuko...lld:pubmed
pubmed-article:12481100pubmed:authorpubmed-author:Inohara-Ochia...lld:pubmed
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pubmed-article:12481100pubmed:volume130lld:pubmed
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pubmed-article:12481100pubmed:pagination2164-76lld:pubmed
pubmed-article:12481100pubmed:dateRevised2010-9-14lld:pubmed
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