| pubmed-article:12435689 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12435689 | lifeskim:mentions | umls-concept:C0019704 | lld:lifeskim |
| pubmed-article:12435689 | lifeskim:mentions | umls-concept:C0043474 | lld:lifeskim |
| pubmed-article:12435689 | lifeskim:mentions | umls-concept:C0086972 | lld:lifeskim |
| pubmed-article:12435689 | lifeskim:mentions | umls-concept:C0026882 | lld:lifeskim |
| pubmed-article:12435689 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:12435689 | lifeskim:mentions | umls-concept:C0032520 | lld:lifeskim |
| pubmed-article:12435689 | lifeskim:mentions | umls-concept:C0683598 | lld:lifeskim |
| pubmed-article:12435689 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:12435689 | lifeskim:mentions | umls-concept:C0449560 | lld:lifeskim |
| pubmed-article:12435689 | lifeskim:mentions | umls-concept:C0439831 | lld:lifeskim |
| pubmed-article:12435689 | pubmed:dateCreated | 2002-11-18 | lld:pubmed |
| pubmed-article:12435689 | pubmed:abstractText | A rapid zidovudine (ZDV) resistance genotypic assay was developed based on the mutagenically separated PCR (MS-PCR) technique to detect two ZDV-resistant mutations, M41L and K70R in CRF01_AE (subtype E). Endpoint dilution analysis revealed that the newly constructed MS-PCR assay could successfully detect three to nine copies of human immunodeficiency virus type 1 template RNA. The test against wild-type and mutant template mixtures in different ratios demonstrated that the assay could detect 10% minor population, at least. Fifty-one subtype E clinical samples were analyzed by the newly constructed MS-PCR assay and direct nucleotide sequencing. The concordance of the two assays was 92 and 100% in codons 41 and 70, respectively. The MS-PCR assay is a rapid, simple, and inexpensive assay that is highly sensitive in detecting mutant targets, including minor populations. Thus, it could be used as a powerful tool for epidemiological surveillance of drug-resistant mutations in developing countries. | lld:pubmed |
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| pubmed-article:12435689 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12435689 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:12435689 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12435689 | pubmed:author | pubmed-author:AuwanitWattan... | lld:pubmed |
| pubmed-article:12435689 | pubmed:author | pubmed-author:SugiuraWataru... | lld:pubmed |
| pubmed-article:12435689 | pubmed:author | pubmed-author:AriyoshiKoyaK | lld:pubmed |
| pubmed-article:12435689 | pubmed:author | pubmed-author:MatsudaMasaka... | lld:pubmed |
| pubmed-article:12435689 | pubmed:author | pubmed-author:YamadaKaneoK | lld:pubmed |
| pubmed-article:12435689 | pubmed:author | pubmed-author:WeberJonathan... | lld:pubmed |
| pubmed-article:12435689 | pubmed:author | pubmed-author:FraterAlexand... | lld:pubmed |
| pubmed-article:12435689 | pubmed:author | pubmed-author:ChibaTomokoT | lld:pubmed |
| pubmed-article:12435689 | pubmed:author | pubmed-author:YanHuaH | lld:pubmed |
| pubmed-article:12435689 | pubmed:author | pubmed-author:MyintLayL | lld:pubmed |
| pubmed-article:12435689 | pubmed:author | pubmed-author:PathipvanithP... | lld:pubmed |
| pubmed-article:12435689 | pubmed:author | pubmed-author:FujitaKazunor... | lld:pubmed |
| pubmed-article:12435689 | pubmed:author | pubmed-author:McClureMyraM | lld:pubmed |
| pubmed-article:12435689 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12435689 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12435689 | pubmed:pagination | 3861-8 | lld:pubmed |
| pubmed-article:12435689 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:12435689 | pubmed:articleTitle | Mutagenically separated PCR assay for rapid detection of M41L and K70R zidovudine resistance mutations in CRF01_AE (subtype E) human immunodeficiency virus type 1. | lld:pubmed |
| pubmed-article:12435689 | pubmed:affiliation | AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan. | lld:pubmed |
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