| pubmed-article:12388315 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12388315 | lifeskim:mentions | umls-concept:C0150369 | lld:lifeskim |
| pubmed-article:12388315 | lifeskim:mentions | umls-concept:C0014257 | lld:lifeskim |
| pubmed-article:12388315 | lifeskim:mentions | umls-concept:C0205042 | lld:lifeskim |
| pubmed-article:12388315 | lifeskim:mentions | umls-concept:C0178719 | lld:lifeskim |
| pubmed-article:12388315 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:12388315 | lifeskim:mentions | umls-concept:C0444498 | lld:lifeskim |
| pubmed-article:12388315 | lifeskim:mentions | umls-concept:C0521115 | lld:lifeskim |
| pubmed-article:12388315 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:12388315 | pubmed:dateCreated | 2002-11-12 | lld:pubmed |
| pubmed-article:12388315 | pubmed:abstractText | We developed an in situ assay system to simultaneously monitor intracellular Ca(2+) concentration ([Ca(2+)](i), fura 2 as indicator) and nitric oxide (NO) levels [4,5-diaminofluorescein as probe] in the intact endothelium of small bovine coronary arteries by using a fluorescent microscopic imaging technique with high-speed wavelength switching. Bradykinin (BK; 1 microM) stimulated a rapid increase in [Ca(2+)](i) followed by an increase in NO production in the endothelial cells. The protein tyrosine phosphatase inhibitor phenylarsine oxide (PAO; 10 microM) induced a gradual, small increase in [Ca(2+)](i) and a slow increase in intracellular NO levels. Removal of extracellular Ca(2+) and depletion of Ca(2+) stores completely blocked BK-induced increase in NO production but had no effect on PAO-induced NO production. However, a further reduction of [Ca(2+)](i) by application of BAPTA-AM or EGTA with ionomycin abolished the PAO-induced NO increase. These results indicate that a simultaneous monitoring of [Ca(2+)](i) and intracellular NO production in the intact endothelium is a powerful tool to study Ca(2+)-dependent regulation of endothelial nitric oxide synthase, which provides the first direct evidence for a permissive role of Ca(2+) in tyrosine phosphorylation-induced NO production. | lld:pubmed |
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| pubmed-article:12388315 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12388315 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12388315 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:12388315 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12388315 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:12388315 | pubmed:issn | 0363-6135 | lld:pubmed |
| pubmed-article:12388315 | pubmed:author | pubmed-author:CampbellWilli... | lld:pubmed |
| pubmed-article:12388315 | pubmed:author | pubmed-author:ZouAi-PingAP | lld:pubmed |
| pubmed-article:12388315 | pubmed:author | pubmed-author:LiPin-LanPL | lld:pubmed |
| pubmed-article:12388315 | pubmed:author | pubmed-author:ZhangAndrew... | lld:pubmed |
| pubmed-article:12388315 | pubmed:author | pubmed-author:YiFu-XianFX | lld:pubmed |
| pubmed-article:12388315 | pubmed:author | pubmed-author:Van... | lld:pubmed |
| pubmed-article:12388315 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12388315 | pubmed:volume | 283 | lld:pubmed |
| pubmed-article:12388315 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12388315 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12388315 | pubmed:pagination | H2725-32 | lld:pubmed |
| pubmed-article:12388315 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
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| pubmed-article:12388315 | pubmed:meshHeading | pubmed-meshheading:12388315... | lld:pubmed |
| pubmed-article:12388315 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12388315 | pubmed:articleTitle | Simultaneous in situ monitoring of intracellular Ca2+ and NO in endothelium of coronary arteries. | lld:pubmed |
| pubmed-article:12388315 | pubmed:affiliation | Department of Pharmacology, Medical College of Wisconsin, Milwaukee 53226, USA. | lld:pubmed |
| pubmed-article:12388315 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12388315 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:12388315 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:12388315 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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