| pubmed-article:12374773 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12374773 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:12374773 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:12374773 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:12374773 | lifeskim:mentions | umls-concept:C0016030 | lld:lifeskim |
| pubmed-article:12374773 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:12374773 | lifeskim:mentions | umls-concept:C1514559 | lld:lifeskim |
| pubmed-article:12374773 | lifeskim:mentions | umls-concept:C0250414 | lld:lifeskim |
| pubmed-article:12374773 | lifeskim:mentions | umls-concept:C0205160 | lld:lifeskim |
| pubmed-article:12374773 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:12374773 | lifeskim:mentions | umls-concept:C1414314 | lld:lifeskim |
| pubmed-article:12374773 | lifeskim:mentions | umls-concept:C0220905 | lld:lifeskim |
| pubmed-article:12374773 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:12374773 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:12374773 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:12374773 | pubmed:dateCreated | 2002-10-10 | lld:pubmed |
| pubmed-article:12374773 | pubmed:abstractText | The early growth response 1 (Egr-1) gene product is a transcription factor that functions as an oikis factor. Loss of Egr-1 expression is closely associated with tumor formation. Phospholipase Cgamma1 (PLCgamma1) is overexpressed in some tumors, and its overexpression causes anchorage-independent growth. Here we report that overexpression of PLCgamma1 and SH2-SH3 domain of PLCgamma1 decreased induction of Egr-1 and the Egr-1-regulated genes TSP-1 and PAI-1. Results from the nuclear run-on assay and transfection experiment with the proximal 455 base pair region of the Egr-1 promoter (-454 to +1) showed that Egr-1 transcriptional activity was suppressed in PLCgamma1-3Y1 cells whereas decay of Egr-1 mRNA was similar in both cell lines. Serum response element- and ternary complex factor Elk-1-mediated transcriptional activation of the reporter gene in response to EGF were also inhibited in PLCgamma1-3Y1 cells. Pretreatment with the protein synthesis inhibitor cycloheximide (CHX) partially abrogated the serum-induced suppression of Egr-1 transcription in PLCgamma1-3Y1 cells, suggesting that a CHX-sensitive factor(s) is involved in the suppression of Egr-1 transcription in PLCgamma1-3Y1 cells. Our results demonstrated that overexpression of PLCgamma1 functions as a negative modulator of the tumor suppressor Egr-1 gene expression, possibly through inhibition of Elk-1-dependent transcriptional activity. | lld:pubmed |
| pubmed-article:12374773 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12374773 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12374773 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12374773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12374773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12374773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12374773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12374773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12374773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12374773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12374773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12374773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12374773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12374773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12374773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12374773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12374773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12374773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12374773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12374773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12374773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12374773 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12374773 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:12374773 | pubmed:issn | 1530-6860 | lld:pubmed |
| pubmed-article:12374773 | pubmed:author | pubmed-author:KIMO SOS | lld:pubmed |
| pubmed-article:12374773 | pubmed:author | pubmed-author:NaDoe SunDS | lld:pubmed |
| pubmed-article:12374773 | pubmed:author | pubmed-author:ShinSoon... | lld:pubmed |
| pubmed-article:12374773 | pubmed:author | pubmed-author:LeeYoung... | lld:pubmed |
| pubmed-article:12374773 | pubmed:author | pubmed-author:SuhPann-Ghill... | lld:pubmed |
| pubmed-article:12374773 | pubmed:author | pubmed-author:MinDo SikDS | lld:pubmed |
| pubmed-article:12374773 | pubmed:author | pubmed-author:HanMi YoungMY | lld:pubmed |
| pubmed-article:12374773 | pubmed:author | pubmed-author:KimYong SikYS | lld:pubmed |
| pubmed-article:12374773 | pubmed:author | pubmed-author:ChangJong-Soo... | lld:pubmed |
| pubmed-article:12374773 | pubmed:author | pubmed-author:BaeSun SikSS | lld:pubmed |
| pubmed-article:12374773 | pubmed:author | pubmed-author:KimYoung-HoYH | lld:pubmed |
| pubmed-article:12374773 | pubmed:author | pubmed-author:KoJesangJ | lld:pubmed |
| pubmed-article:12374773 | pubmed:author | pubmed-author:KimMyung... | lld:pubmed |
| pubmed-article:12374773 | pubmed:author | pubmed-author:HyunDae... | lld:pubmed |
| pubmed-article:12374773 | pubmed:author | pubmed-author:KimJung-HyeJH | lld:pubmed |
| pubmed-article:12374773 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:12374773 | pubmed:volume | 16 | lld:pubmed |
| pubmed-article:12374773 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12374773 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12374773 | pubmed:pagination | 1504-14 | lld:pubmed |
| pubmed-article:12374773 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:12374773 | pubmed:meshHeading | pubmed-meshheading:12374773... | lld:pubmed |
| pubmed-article:12374773 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12374773 | pubmed:articleTitle | Negative regulatory role of overexpression of PLC gamma 1 in the expression of early growth response 1 gene in rat 3Y1 fibroblasts. | lld:pubmed |
| pubmed-article:12374773 | pubmed:affiliation | Department of Biochemistry and Molecular Biology, College of Medicine, Yeungnam University, Daegu, Korea 705-717. | lld:pubmed |
| pubmed-article:12374773 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12374773 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
