| pubmed-article:12220625 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12220625 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:12220625 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:12220625 | lifeskim:mentions | umls-concept:C0205065 | lld:lifeskim |
| pubmed-article:12220625 | lifeskim:mentions | umls-concept:C0087071 | lld:lifeskim |
| pubmed-article:12220625 | lifeskim:mentions | umls-concept:C1155299 | lld:lifeskim |
| pubmed-article:12220625 | lifeskim:mentions | umls-concept:C0021467 | lld:lifeskim |
| pubmed-article:12220625 | lifeskim:mentions | umls-concept:C0021469 | lld:lifeskim |
| pubmed-article:12220625 | lifeskim:mentions | umls-concept:C0205197 | lld:lifeskim |
| pubmed-article:12220625 | pubmed:issue | 1-3 | lld:pubmed |
| pubmed-article:12220625 | pubmed:dateCreated | 2002-9-10 | lld:pubmed |
| pubmed-article:12220625 | pubmed:abstractText | The two known mechanisms for telomere maintenance in eukaryocytes are telomerase in telomerase-positive cells and alternative lengthening of telomeres (ALT) in telomerase-negative cells. We report here that telomere maintenance in the telomerase-positive human ovarian SKOV-3 cells was not affected by inhibition of telomerase. For comparison, the effect of telomerase inhibitors on telomere maintenance in another telomerase-positive cell line (i.e. human pharynx FaDu cells) and the telomerase-negative human osteosarcoma Saos-2 cells was examined. Telomerase activity was measured using a modified telomeric repeat amplification protocol and telomere length was measured using a solution hybridization-based method and fluorescence in situ hybridization. A reverse transcriptase inhibitor (3'-azido-deoxythymidine or AZT) and an antisense against a component of human telomerase RNA (antisense hTR) were used to inhibit telomerase. FaDu and SKOV-3 cells showed comparable baseline telomerase activity. Telomerase activity in both cells was inhibited about equally by AZT (maximal inhibition of approximately 80%) and by expression of antisense hTR (complete inhibition in SKOV-3 cells and maximal inhibition of approximately 80% in FaDu cells). However, treatment with telomerase inhibitors resulted in approximately 50% telomere shortening in FaDu cells but had no effect on SKOV-3 nor Saos-2 cells. SKOV-3 cells did not show the characteristic features of ALT (i.e. heterogeneous telomere length and promyelocytic leukemia bodies), whereas these ALT features were observed in Saos-2 cells. Collectively, these results suggest the existence of a telomerase-independent mechanism of telomere maintenance in the telomerase-positive SKOV-3 cells. | lld:pubmed |
| pubmed-article:12220625 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12220625 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12220625 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12220625 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12220625 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12220625 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12220625 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12220625 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12220625 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12220625 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:12220625 | pubmed:issn | 0014-5793 | lld:pubmed |
| pubmed-article:12220625 | pubmed:author | pubmed-author:AuJessie... | lld:pubmed |
| pubmed-article:12220625 | pubmed:author | pubmed-author:LuJieJ | lld:pubmed |
| pubmed-article:12220625 | pubmed:author | pubmed-author:GanYueboY | lld:pubmed |
| pubmed-article:12220625 | pubmed:author | pubmed-author:MoYiqunY | lld:pubmed |
| pubmed-article:12220625 | pubmed:author | pubmed-author:JohnstonJeffr... | lld:pubmed |
| pubmed-article:12220625 | pubmed:author | pubmed-author:WientjesM... | lld:pubmed |
| pubmed-article:12220625 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12220625 | pubmed:day | 11 | lld:pubmed |
| pubmed-article:12220625 | pubmed:volume | 527 | lld:pubmed |
| pubmed-article:12220625 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12220625 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12220625 | pubmed:pagination | 10-4 | lld:pubmed |
| pubmed-article:12220625 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:12220625 | pubmed:meshHeading | pubmed-meshheading:12220625... | lld:pubmed |
| pubmed-article:12220625 | pubmed:meshHeading | pubmed-meshheading:12220625... | lld:pubmed |
| pubmed-article:12220625 | pubmed:meshHeading | pubmed-meshheading:12220625... | lld:pubmed |
| pubmed-article:12220625 | pubmed:meshHeading | pubmed-meshheading:12220625... | lld:pubmed |
| pubmed-article:12220625 | pubmed:meshHeading | pubmed-meshheading:12220625... | lld:pubmed |
| pubmed-article:12220625 | pubmed:meshHeading | pubmed-meshheading:12220625... | lld:pubmed |
| pubmed-article:12220625 | pubmed:meshHeading | pubmed-meshheading:12220625... | lld:pubmed |
| pubmed-article:12220625 | pubmed:meshHeading | pubmed-meshheading:12220625... | lld:pubmed |
| pubmed-article:12220625 | pubmed:meshHeading | pubmed-meshheading:12220625... | lld:pubmed |
| pubmed-article:12220625 | pubmed:meshHeading | pubmed-meshheading:12220625... | lld:pubmed |
| pubmed-article:12220625 | pubmed:meshHeading | pubmed-meshheading:12220625... | lld:pubmed |
| pubmed-article:12220625 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12220625 | pubmed:articleTitle | Telomere maintenance in telomerase-positive human ovarian SKOV-3 cells cannot be retarded by complete inhibition of telomerase. | lld:pubmed |
| pubmed-article:12220625 | pubmed:affiliation | College of Pharmacy, The Ohio State University, 500 West 12th Avenue, Columbus, OH 43210, USA. | lld:pubmed |
| pubmed-article:12220625 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12220625 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12220625 | lld:pubmed |
