| pubmed-article:12145339 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12145339 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:12145339 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:12145339 | lifeskim:mentions | umls-concept:C0038323 | lld:lifeskim |
| pubmed-article:12145339 | lifeskim:mentions | umls-concept:C0023025 | lld:lifeskim |
| pubmed-article:12145339 | lifeskim:mentions | umls-concept:C0005456 | lld:lifeskim |
| pubmed-article:12145339 | lifeskim:mentions | umls-concept:C1413892 | lld:lifeskim |
| pubmed-article:12145339 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:12145339 | lifeskim:mentions | umls-concept:C0013879 | lld:lifeskim |
| pubmed-article:12145339 | lifeskim:mentions | umls-concept:C0205224 | lld:lifeskim |
| pubmed-article:12145339 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:12145339 | pubmed:dateCreated | 2002-7-29 | lld:pubmed |
| pubmed-article:12145339 | pubmed:abstractText | Lanosterol 14alpha-demethylase (CYP51) is involved in the cholesterol biosynthesis pathway, producing follicular fluid meiosis-activating sterol. The promoter region of the human CYP51 gene contains a cluster of regulatory elements including GC box, cAMP response element (CRE), and sterol regulatory element (SRE). To understand the mechanism of sterol-dependent regulation of this gene, several constructs of the promoter with the reporter gene have been tested in JEG-3 cells containing overexpressed human sterol regulatory element binding protein (SREBP)-1a. The wild-type construct showed maximal SREBP-dependent activation, most of which is retained when the GC box is mutated/deleted. Activation is abolished when either CRE or SRE are removed/mutated. Furthermore, mutation of CRE abolishes SREBP-dependent activation after overexpression of SREBP-1a and CRE binding protein (CREB). This shows that CRE is essential, and that under ex vivo conditions CREB and SREBP cooperate in transactivating CYP51. Interestingly, protein kinase A shows a marked stimulation of the CYP51 promoter activity when overexpressed together with SREBP-1a but not when overexpressed with CREB, suggesting phosphorylation of SREBP-1a. Using a DNA probe containing all three regulatory elements, it is found that SREBP-1a, a CREB-like factor, and specificity protein (Sp1) all probably bind the CYP51 promoter. While SREBP-1a and the CRE-bound proteins are essential for the SREBP-dependent response, Sp1 apparently functions only to maximize sterol regulation of CYP51. To date this is the first gene in which cooperation between SREBP and a CREB/CRE modulator/activating transcription factor family transcription factor is shown to be essential and sufficient for SREBP-dependent activation. | lld:pubmed |
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| pubmed-article:12145339 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12145339 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12145339 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:12145339 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12145339 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:12145339 | pubmed:issn | 0888-8809 | lld:pubmed |
| pubmed-article:12145339 | pubmed:author | pubmed-author:RozmanDamjana... | lld:pubmed |
| pubmed-article:12145339 | pubmed:author | pubmed-author:WatermanMicha... | lld:pubmed |
| pubmed-article:12145339 | pubmed:author | pubmed-author:HalderSunil... | lld:pubmed |
| pubmed-article:12145339 | pubmed:author | pubmed-author:FinkMartinaM | lld:pubmed |
| pubmed-article:12145339 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12145339 | pubmed:volume | 16 | lld:pubmed |
| pubmed-article:12145339 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12145339 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12145339 | pubmed:pagination | 1853-63 | lld:pubmed |
| pubmed-article:12145339 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:12145339 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12145339 | pubmed:articleTitle | A cAMP-responsive element binding site is essential for sterol regulation of the human lanosterol 14alpha-demethylase gene (CYP51). | lld:pubmed |
| pubmed-article:12145339 | pubmed:affiliation | Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0146, USA. | lld:pubmed |
| pubmed-article:12145339 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12145339 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:12145339 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:12145339 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:12145339 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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