| pubmed-article:12110315 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12110315 | lifeskim:mentions | umls-concept:C0028630 | lld:lifeskim |
| pubmed-article:12110315 | lifeskim:mentions | umls-concept:C0028621 | lld:lifeskim |
| pubmed-article:12110315 | lifeskim:mentions | umls-concept:C0205360 | lld:lifeskim |
| pubmed-article:12110315 | lifeskim:mentions | umls-concept:C0332256 | lld:lifeskim |
| pubmed-article:12110315 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:12110315 | pubmed:dateCreated | 2002-7-11 | lld:pubmed |
| pubmed-article:12110315 | pubmed:abstractText | In order to develop novel antigene molecules forming thermally stable triplexes with target DNAs and having nuclease resistance properties, we synthesized oligodeoxynucleotides (ODNs) with various lengths of aminoalkyl-linkers at the 4'alpha position of thymidine and the aminoethyl-linker at the 4'alpha position of 2'-deoxy-5-methylcytidine. Thermal stability of triplexes between these ODNs and a DNA duplex was studied by thermal denaturation. The ODNs containing the nucleoside 2 with the aminoethyl-linker or the nucleoside 3 with the aminopropyl-linker thermally stabilized the triplexes, whereas the ODNs containing the nucleoside 1 with the aminomethyl-linker or the nucleoside 4 with the 2-[N-(2-aminoethyl)carbamoyl]oxy]ethyl-linker thermally destabilized the triplexes. The ODNs containing 2 were the most efficient at stabilizing the triplexes with the target DNA. The ODNs containing 4'alpha-C-(2-aminoethyl)-2'-deoxy-5-methylcytidine (5) also efficiently stabilized the triplexes with the target DNA. Stability of the ODN containing 5 to nucleolytic hydrolysis by snake venom phosphodiesterase (a 3'-exonuclease) was studied. It was found that the ODN containing 5 was more resistant to nucleolytic digestion by the enzyme than an unmodified ODN. In a previous paper, we reported that the ODNs containing 2 were more resistant to nucleolytic digestion by DNase I (an endonuclease) than the unmodified ODNs. Thus, it was found that the ODNs containing 4'alpha-C-(2-aminoethyl)-2'-deoxynucleosides were good candidates for antigene molecules. | lld:pubmed |
| pubmed-article:12110315 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12110315 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12110315 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12110315 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12110315 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12110315 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12110315 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12110315 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12110315 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12110315 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12110315 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12110315 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12110315 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:12110315 | pubmed:issn | 0968-0896 | lld:pubmed |
| pubmed-article:12110315 | pubmed:author | pubmed-author:UenoYoshihito... | lld:pubmed |
| pubmed-article:12110315 | pubmed:author | pubmed-author:MatsudaAkiraA | lld:pubmed |
| pubmed-article:12110315 | pubmed:author | pubmed-author:ShutoSatoshiS | lld:pubmed |
| pubmed-article:12110315 | pubmed:author | pubmed-author:AtsumiNaokoN | lld:pubmed |
| pubmed-article:12110315 | pubmed:author | pubmed-author:KanazakiMakik... | lld:pubmed |
| pubmed-article:12110315 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12110315 | pubmed:volume | 10 | lld:pubmed |
| pubmed-article:12110315 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12110315 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12110315 | pubmed:pagination | 2933-9 | lld:pubmed |
| pubmed-article:12110315 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:12110315 | pubmed:meshHeading | pubmed-meshheading:12110315... | lld:pubmed |
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| pubmed-article:12110315 | pubmed:meshHeading | pubmed-meshheading:12110315... | lld:pubmed |
| pubmed-article:12110315 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12110315 | pubmed:articleTitle | Nucleosides and nucleotides. Part 214: thermal stability of triplexes containing 4'alpha-C-aminoalkyl-2'-deoxynucleosides. | lld:pubmed |
| pubmed-article:12110315 | pubmed:affiliation | Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan. | lld:pubmed |
| pubmed-article:12110315 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12110315 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
