| pubmed-article:12052729 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12052729 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:12052729 | lifeskim:mentions | umls-concept:C0237868 | lld:lifeskim |
| pubmed-article:12052729 | lifeskim:mentions | umls-concept:C0577559 | lld:lifeskim |
| pubmed-article:12052729 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:12052729 | lifeskim:mentions | umls-concept:C1521991 | lld:lifeskim |
| pubmed-article:12052729 | lifeskim:mentions | umls-concept:C0036679 | lld:lifeskim |
| pubmed-article:12052729 | lifeskim:mentions | umls-concept:C1705920 | lld:lifeskim |
| pubmed-article:12052729 | lifeskim:mentions | umls-concept:C0301704 | lld:lifeskim |
| pubmed-article:12052729 | lifeskim:mentions | umls-concept:C0596837 | lld:lifeskim |
| pubmed-article:12052729 | lifeskim:mentions | umls-concept:C0008565 | lld:lifeskim |
| pubmed-article:12052729 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:12052729 | pubmed:dateCreated | 2002-6-7 | lld:pubmed |
| pubmed-article:12052729 | pubmed:abstractText | A reversed-phase HPLC method compatible with evaporative light scattering (ELS) and electrospray mass spectrometric (ES-MS) detection was developed for separation of phosphatidylserine (PS) molecular species. The method was optimised for separation of three disaturated synthetic species: dipalmitoyl glycerophosphoserine, palmitoyl-stearoyl glycerophosphoserine and distearoyl glycerophosphoserine using isocratic elution with a mixture of 2-propanol, tetrahydrofuran and ammonium formate. Baseline separation was obtained on three different columns: one polystyrene/divinylbenzene (PS/DVB) column and two silica based C(18) and C(30) columns. The best chromatographic resolution was achieved with the C(30) column. The limit of detection for DPPS was 5 microg/ml (S/N=3) with ELS detection and 0.1 microg/ml (S/N=3) with negative ion ES-MS in the single ion monitoring mode. Baseline separation of the five main species in a biological PS sample, bovine brain PS, was obtained with the PS/DVB column. Species identification was done by using the retention times of the intact PS species and their corresponding carboxylate anion fragments obtained by in-source fragmentation. Data have shown that individual PS species can be identified by their retention times using direct ELS detection in a mixture of disaturated PS species. However, for the bovine brain PS electrospray-MS detection was necessary for species identification due to the many possible fatty acid combinations in biological PS. | lld:pubmed |
| pubmed-article:12052729 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12052729 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12052729 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12052729 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12052729 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12052729 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:12052729 | pubmed:issn | 1570-0232 | lld:pubmed |
| pubmed-article:12052729 | pubmed:author | pubmed-author:LundanesElsaE | lld:pubmed |
| pubmed-article:12052729 | pubmed:author | pubmed-author:HvattumErlend... | lld:pubmed |
| pubmed-article:12052729 | pubmed:author | pubmed-author:LarsenAsmundA | lld:pubmed |
| pubmed-article:12052729 | pubmed:author | pubmed-author:MokastetEvaE | lld:pubmed |
| pubmed-article:12052729 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12052729 | pubmed:day | 5 | lld:pubmed |
| pubmed-article:12052729 | pubmed:volume | 774 | lld:pubmed |
| pubmed-article:12052729 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12052729 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12052729 | pubmed:pagination | 115-20 | lld:pubmed |
| pubmed-article:12052729 | pubmed:dateRevised | 2003-11-14 | lld:pubmed |
| pubmed-article:12052729 | pubmed:meshHeading | pubmed-meshheading:12052729... | lld:pubmed |
| pubmed-article:12052729 | pubmed:meshHeading | pubmed-meshheading:12052729... | lld:pubmed |
| pubmed-article:12052729 | pubmed:meshHeading | pubmed-meshheading:12052729... | lld:pubmed |
| pubmed-article:12052729 | pubmed:meshHeading | pubmed-meshheading:12052729... | lld:pubmed |
| pubmed-article:12052729 | pubmed:meshHeading | pubmed-meshheading:12052729... | lld:pubmed |
| pubmed-article:12052729 | pubmed:meshHeading | pubmed-meshheading:12052729... | lld:pubmed |
| pubmed-article:12052729 | pubmed:meshHeading | pubmed-meshheading:12052729... | lld:pubmed |
| pubmed-article:12052729 | pubmed:meshHeading | pubmed-meshheading:12052729... | lld:pubmed |
| pubmed-article:12052729 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12052729 | pubmed:articleTitle | Separation and identification of phosphatidylserine molecular species using reversed-phase high-performance liquid chromatography with evaporative light scattering and mass spectrometric detection. | lld:pubmed |
| pubmed-article:12052729 | pubmed:affiliation | Department of Chemistry, University of Oslo, PO Box 1033, Blindern, 0315 Oslo, Norway. aasmunl@kjemi.uio.no | lld:pubmed |
| pubmed-article:12052729 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12052729 | lld:pubmed |
