| pubmed-article:12021115 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12021115 | lifeskim:mentions | umls-concept:C0011854 | lld:lifeskim |
| pubmed-article:12021115 | lifeskim:mentions | umls-concept:C0004358 | lld:lifeskim |
| pubmed-article:12021115 | lifeskim:mentions | umls-concept:C0242485 | lld:lifeskim |
| pubmed-article:12021115 | lifeskim:mentions | umls-concept:C1419119 | lld:lifeskim |
| pubmed-article:12021115 | lifeskim:mentions | umls-concept:C0014441 | lld:lifeskim |
| pubmed-article:12021115 | pubmed:dateCreated | 2002-5-21 | lld:pubmed |
| pubmed-article:12021115 | pubmed:abstractText | The tyrosine phosphatase-like protein IA-2 is an important islet autoantigen in type 1 diabetes. Although the radioligand binding assay with in vitro synthesized (35)S-labeled antigen has been used extensively for measuring autoantibodies to IA-2, disadvantages of both assays with respect to synthesis and handling of the radioactive antigen limit their use in routine laboratories. Therefore, we attempted to develop a nonradioactive ELISA for the simple detection of IA-2 autoantibodies. The biotinylated cytoplasmic domain of IA-2 expressed in Escherichia coli was used as an antigen. We evaluated two kinds of ELISA: ELISA with biotin-IA-2 directly captured on streptavidine-coated plates (solid phase) and ELISA with antigen-antibody preincubation in solution in which serum samples were reacted first with biotin-IA-2 and the mixture was transferred to streptavidine-coated plates (liquid phase). We compared their disease sensitivity and specificity with a conventional radioligand binding assay in 52 patients with recent-onset type 1 diabetes and 138 normal individuals. The radioligand binding assay had 61.5% sensitivity and 99.3% specificity. The liquid-phase ELISA showed relatively higher sensitivity (55.8%) and specificity (99.3%) than the solid-phase ELISA (sensitivity 53.8% and specificity 97.1%). Furthermore, the mean SD score in IA-2 autoantibody-positive serum samples measured by liquid-phase ELISA was significantly higher than the SD score obtained by solid-phase ELISA (P < 0.0001). We concluded that this liquid-phase ELISA is suitable for detecting IA-2 autoantibodies in patients with type 1 diabetes with a similar sensitivity and specificity to those of conventional radioligand binding assay. | lld:pubmed |
| pubmed-article:12021115 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12021115 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12021115 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12021115 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12021115 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12021115 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12021115 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:12021115 | pubmed:issn | 0077-8923 | lld:pubmed |
| pubmed-article:12021115 | pubmed:author | pubmed-author:HattoriHiroak... | lld:pubmed |
| pubmed-article:12021115 | pubmed:author | pubmed-author:EgashiraToruT | lld:pubmed |
| pubmed-article:12021115 | pubmed:author | pubmed-author:EguchiKatsumi... | lld:pubmed |
| pubmed-article:12021115 | pubmed:author | pubmed-author:YamaguchiHiro... | lld:pubmed |
| pubmed-article:12021115 | pubmed:author | pubmed-author:KawasakiEijiE | lld:pubmed |
| pubmed-article:12021115 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12021115 | pubmed:volume | 958 | lld:pubmed |
| pubmed-article:12021115 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12021115 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12021115 | pubmed:pagination | 241-6 | lld:pubmed |
| pubmed-article:12021115 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
| pubmed-article:12021115 | pubmed:meshHeading | pubmed-meshheading:12021115... | lld:pubmed |
| pubmed-article:12021115 | pubmed:meshHeading | pubmed-meshheading:12021115... | lld:pubmed |
| pubmed-article:12021115 | pubmed:meshHeading | pubmed-meshheading:12021115... | lld:pubmed |
| pubmed-article:12021115 | pubmed:meshHeading | pubmed-meshheading:12021115... | lld:pubmed |
| pubmed-article:12021115 | pubmed:meshHeading | pubmed-meshheading:12021115... | lld:pubmed |
| pubmed-article:12021115 | pubmed:meshHeading | pubmed-meshheading:12021115... | lld:pubmed |
| pubmed-article:12021115 | pubmed:meshHeading | pubmed-meshheading:12021115... | lld:pubmed |
| pubmed-article:12021115 | pubmed:meshHeading | pubmed-meshheading:12021115... | lld:pubmed |
| pubmed-article:12021115 | pubmed:meshHeading | pubmed-meshheading:12021115... | lld:pubmed |
| pubmed-article:12021115 | pubmed:meshHeading | pubmed-meshheading:12021115... | lld:pubmed |
| pubmed-article:12021115 | pubmed:meshHeading | pubmed-meshheading:12021115... | lld:pubmed |
| pubmed-article:12021115 | pubmed:meshHeading | pubmed-meshheading:12021115... | lld:pubmed |
| pubmed-article:12021115 | pubmed:meshHeading | pubmed-meshheading:12021115... | lld:pubmed |
| pubmed-article:12021115 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12021115 | pubmed:articleTitle | Autoantibodies to IA-2 in type 1 diabetes: measurements with a new enzyme-linked immunosorbent assay. | lld:pubmed |
| pubmed-article:12021115 | pubmed:affiliation | The First Department of Internal Medicine, Nagasaki University School of Medicine, Nagasaki 852-8501, Japan. f1196@cc.nagasaki-u.ac.jp | lld:pubmed |
| pubmed-article:12021115 | pubmed:publicationType | Journal Article | lld:pubmed |
| entrez-gene:5798 | entrezgene:pubmed | pubmed-article:12021115 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:12021115 | lld:entrezgene |
