pubmed-article:12004062 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:12004062 | lifeskim:mentions | umls-concept:C1123023 | lld:lifeskim |
pubmed-article:12004062 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
pubmed-article:12004062 | lifeskim:mentions | umls-concept:C0030946 | lld:lifeskim |
pubmed-article:12004062 | lifeskim:mentions | umls-concept:C0145947 | lld:lifeskim |
pubmed-article:12004062 | lifeskim:mentions | umls-concept:C0333516 | lld:lifeskim |
pubmed-article:12004062 | lifeskim:mentions | umls-concept:C0597304 | lld:lifeskim |
pubmed-article:12004062 | lifeskim:mentions | umls-concept:C1413809 | lld:lifeskim |
pubmed-article:12004062 | lifeskim:mentions | umls-concept:C0332298 | lld:lifeskim |
pubmed-article:12004062 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
pubmed-article:12004062 | lifeskim:mentions | umls-concept:C0127400 | lld:lifeskim |
pubmed-article:12004062 | pubmed:issue | 30 | lld:pubmed |
pubmed-article:12004062 | pubmed:dateCreated | 2002-7-22 | lld:pubmed |
pubmed-article:12004062 | pubmed:abstractText | The proteolytic activation of pro-matrix metalloproteinase (MMP)-9 by conversion of the 92-kDa precursor into an 82-kDa active form has been observed in chronic wounds, tumor metastasis, and many inflammation-associated diseases, yet the mechanistic pathway to control this process has not been identified. In this report, we show that the massive expression and activation of MMP-9 in skin tissue from patients with chronically unhealed wounds could be reconstituted in vitro with cultured normal human skin by stimulation with transforming growth factor-beta and tumor necrosis factor (TNF)-alpha. We dissected the mechanistic pathway for TNF-alpha induced activation of pro-MMP-9 in human skin. We found that proteolytic activation of pro-MMP-9 was mediated by a tissue-associated chymotrypsin-like proteinase, designated here as pro-MMP-9 activator (pM9A). This unidentified activator specifically converted pro-MMP-9 but not pro-MMP-2, another member of the gelatinase family. The tissue-bound pM9A was steadily expressed and not regulated by TNF-alpha, which indicated that the cytokine-mediated activation of pro-MMP-9 might be regulated at the inhibitor level. Indeed, the skin constantly secreted tissue inhibitor of metalloproteinase-1 at the basal state. TNF-alpha, but not transforming growth factor-beta, down-regulated this inhibitor. The TNF-alpha-mediated activation of pro-MMP-9 was tightly associated with down-regulation of tissue inhibitor of metalloproteinase-1 in a dose-dependent manner. To establish this linkage, we demonstrate that the recombinant tissue inhibitor of metalloproteinase-1 could block the activation of pro-MMP-9 by either the intact skin or skin fractions. Thus, these studies suggest a novel regulation for the proteolytic activation of MMP-9 in human tissue, which is mediated by tissue-bound activator and controlled by down-regulation of a specific inhibitor. | lld:pubmed |
pubmed-article:12004062 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:language | eng | lld:pubmed |
pubmed-article:12004062 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:12004062 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12004062 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:12004062 | pubmed:month | Jul | lld:pubmed |
pubmed-article:12004062 | pubmed:issn | 0021-9258 | lld:pubmed |
pubmed-article:12004062 | pubmed:author | pubmed-author:HanYuan-PingY... | lld:pubmed |
pubmed-article:12004062 | pubmed:author | pubmed-author:NienYih-DarYD | lld:pubmed |
pubmed-article:12004062 | pubmed:author | pubmed-author:GarnerWarren... | lld:pubmed |
pubmed-article:12004062 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:12004062 | pubmed:day | 26 | lld:pubmed |
pubmed-article:12004062 | pubmed:volume | 277 | lld:pubmed |
pubmed-article:12004062 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:12004062 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:12004062 | pubmed:pagination | 27319-27 | lld:pubmed |
pubmed-article:12004062 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
pubmed-article:12004062 | pubmed:meshHeading | pubmed-meshheading:12004062... | lld:pubmed |
pubmed-article:12004062 | pubmed:meshHeading | pubmed-meshheading:12004062... | lld:pubmed |
pubmed-article:12004062 | pubmed:meshHeading | pubmed-meshheading:12004062... | lld:pubmed |
pubmed-article:12004062 | pubmed:meshHeading | pubmed-meshheading:12004062... | lld:pubmed |
pubmed-article:12004062 | pubmed:meshHeading | pubmed-meshheading:12004062... | lld:pubmed |
pubmed-article:12004062 | pubmed:meshHeading | pubmed-meshheading:12004062... | lld:pubmed |
pubmed-article:12004062 | pubmed:meshHeading | pubmed-meshheading:12004062... | lld:pubmed |
pubmed-article:12004062 | pubmed:meshHeading | pubmed-meshheading:12004062... | lld:pubmed |
pubmed-article:12004062 | pubmed:meshHeading | pubmed-meshheading:12004062... | lld:pubmed |
pubmed-article:12004062 | pubmed:meshHeading | pubmed-meshheading:12004062... | lld:pubmed |
pubmed-article:12004062 | pubmed:meshHeading | pubmed-meshheading:12004062... | lld:pubmed |
pubmed-article:12004062 | pubmed:meshHeading | pubmed-meshheading:12004062... | lld:pubmed |
pubmed-article:12004062 | pubmed:meshHeading | pubmed-meshheading:12004062... | lld:pubmed |
pubmed-article:12004062 | pubmed:meshHeading | pubmed-meshheading:12004062... | lld:pubmed |
pubmed-article:12004062 | pubmed:meshHeading | pubmed-meshheading:12004062... | lld:pubmed |
pubmed-article:12004062 | pubmed:meshHeading | pubmed-meshheading:12004062... | lld:pubmed |
pubmed-article:12004062 | pubmed:meshHeading | pubmed-meshheading:12004062... | lld:pubmed |
pubmed-article:12004062 | pubmed:meshHeading | pubmed-meshheading:12004062... | lld:pubmed |
pubmed-article:12004062 | pubmed:year | 2002 | lld:pubmed |