Linked Life Data

11986214

Source:http://linkedlifedata.com/resource/pubmed/id/11986214

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:11986214rdf:typepubmed:Citationlld:pubmed
pubmed-article:11986214lifeskim:mentionsumls-concept:C0020792lld:lifeskim
pubmed-article:11986214lifeskim:mentionsumls-concept:C0036098lld:lifeskim
pubmed-article:11986214lifeskim:mentionsumls-concept:C0040048lld:lifeskim
pubmed-article:11986214lifeskim:mentionsumls-concept:C0021467lld:lifeskim
pubmed-article:11986214lifeskim:mentionsumls-concept:C0015518lld:lifeskim
pubmed-article:11986214lifeskim:mentionsumls-concept:C0040203lld:lifeskim
pubmed-article:11986214lifeskim:mentionsumls-concept:C0164707lld:lifeskim
pubmed-article:11986214lifeskim:mentionsumls-concept:C0439855lld:lifeskim
pubmed-article:11986214lifeskim:mentionsumls-concept:C0015505lld:lifeskim
pubmed-article:11986214lifeskim:mentionsumls-concept:C0015520lld:lifeskim
pubmed-article:11986214lifeskim:mentionsumls-concept:C0021469lld:lifeskim
pubmed-article:11986214lifeskim:mentionsumls-concept:C0679622lld:lifeskim
pubmed-article:11986214lifeskim:mentionsumls-concept:C0205314lld:lifeskim
pubmed-article:11986214lifeskim:mentionsumls-concept:C0282510lld:lifeskim
pubmed-article:11986214pubmed:issue10lld:pubmed
pubmed-article:11986214pubmed:dateCreated2002-5-2lld:pubmed
pubmed-article:11986214pubmed:abstractTextSaliva of the hard tick and Lyme disease vector, Ixodes scapularis, has a repertoire of compounds that counteract host defenses. Following sequencing of an I scapularis salivary gland complementary DNA (cDNA) library, a clone with sequence homology to tissue factor pathway inhibitor (TFPI) was identified. This cDNA codes for a mature protein, herein called Ixolaris, with 140 amino acids containing 10 cysteines and 2 Kunitz-like domains. Recombinant Ixolaris was expressed in insect cells and shown to inhibit factor VIIa (FVIIa)/tissue factor (TF)-induced factor X (FX) activation with an inhibitory concentration of 50% (IC(50)) in the picomolar range. In nondenaturing gel, Ixolaris interacted stoichiometrically with FX and FXa but not FVIIa. Ixolaris behaves as a fast-and-tight ligand of the exosites of FXa and gamma-carboxyglutamic acid domainless FXa (des-Gla-FXa), increasing its amidolytic activity. At high concentration, Ixolaris attenuates the amidolytic activity of FVIIa/TF; however, in the presence of DEGR-FX or DEGR-FXa (but not des-Gla-DEGR-FXa), Ixolaris becomes a tight inhibitor of FVIIa/TF as assessed by recombinant factor IX (BeneFIX) activation assays. This indicates that FX and FXa are scaffolds for Ixolaris in the inhibition of FVIIa/TF and implies that the Gla domain is necessary for FVIIa/TF/Ixolaris/FX(a) complex formation. Additionally, we show that Ixolaris blocks FXa generation by endothelial cells expressing TF. Ixolaris may be a useful tool to study the structural features of FVIIa, FX, and FXa, and an alternative anticoagulant in cardiovascular diseases.lld:pubmed
pubmed-article:11986214pubmed:languageenglld:pubmed
pubmed-article:11986214pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11986214pubmed:citationSubsetAIMlld:pubmed
pubmed-article:11986214pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11986214pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11986214pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11986214pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11986214pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11986214pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11986214pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11986214pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11986214pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11986214pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11986214pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11986214pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11986214pubmed:statusMEDLINElld:pubmed
pubmed-article:11986214pubmed:monthMaylld:pubmed
pubmed-article:11986214pubmed:issn0006-4971lld:pubmed
pubmed-article:11986214pubmed:authorpubmed-author:Francischetti...lld:pubmed
pubmed-article:11986214pubmed:authorpubmed-author:AndersenJohn...lld:pubmed
pubmed-article:11986214pubmed:authorpubmed-author:RibeiroJosé...lld:pubmed
pubmed-article:11986214pubmed:authorpubmed-author:ValenzuelaJes...lld:pubmed
pubmed-article:11986214pubmed:authorpubmed-author:MatherThomas...lld:pubmed
pubmed-article:11986214pubmed:issnTypePrintlld:pubmed
pubmed-article:11986214pubmed:day15lld:pubmed
pubmed-article:11986214pubmed:volume99lld:pubmed
pubmed-article:11986214pubmed:ownerNLMlld:pubmed
pubmed-article:11986214pubmed:authorsCompleteYlld:pubmed
pubmed-article:11986214pubmed:pagination3602-12lld:pubmed
pubmed-article:11986214pubmed:dateRevised2008-11-21lld:pubmed
pubmed-article:11986214pubmed:meshHeadingpubmed-meshheading:11986214...lld:pubmed
pubmed-article:11986214pubmed:meshHeadingpubmed-meshheading:11986214...lld:pubmed
pubmed-article:11986214pubmed:meshHeadingpubmed-meshheading:11986214...lld:pubmed
pubmed-article:11986214pubmed:meshHeadingpubmed-meshheading:11986214...lld:pubmed
pubmed-article:11986214pubmed:meshHeadingpubmed-meshheading:11986214...lld:pubmed
pubmed-article:11986214pubmed:meshHeadingpubmed-meshheading:11986214...lld:pubmed
pubmed-article:11986214pubmed:meshHeadingpubmed-meshheading:11986214...lld:pubmed
pubmed-article:11986214pubmed:meshHeadingpubmed-meshheading:11986214...lld:pubmed
pubmed-article:11986214pubmed:meshHeadingpubmed-meshheading:11986214...lld:pubmed
pubmed-article:11986214pubmed:meshHeadingpubmed-meshheading:11986214...lld:pubmed
pubmed-article:11986214pubmed:meshHeadingpubmed-meshheading:11986214...lld:pubmed
pubmed-article:11986214pubmed:meshHeadingpubmed-meshheading:11986214...lld:pubmed
pubmed-article:11986214pubmed:meshHeadingpubmed-meshheading:11986214...lld:pubmed
pubmed-article:11986214pubmed:meshHeadingpubmed-meshheading:11986214...lld:pubmed
pubmed-article:11986214pubmed:meshHeadingpubmed-meshheading:11986214...lld:pubmed
pubmed-article:11986214pubmed:meshHeadingpubmed-meshheading:11986214...lld:pubmed
pubmed-article:11986214pubmed:meshHeadingpubmed-meshheading:11986214...lld:pubmed
pubmed-article:11986214pubmed:meshHeadingpubmed-meshheading:11986214...lld:pubmed
pubmed-article:11986214pubmed:meshHeadingpubmed-meshheading:11986214...lld:pubmed
pubmed-article:11986214pubmed:meshHeadingpubmed-meshheading:11986214...lld:pubmed
pubmed-article:11986214pubmed:meshHeadingpubmed-meshheading:11986214...lld:pubmed
pubmed-article:11986214pubmed:year2002lld:pubmed
pubmed-article:11986214pubmed:articleTitleIxolaris, a novel recombinant tissue factor pathway inhibitor (TFPI) from the salivary gland of the tick, Ixodes scapularis: identification of factor X and factor Xa as scaffolds for the inhibition of factor VIIa/tissue factor complex.lld:pubmed
pubmed-article:11986214pubmed:affiliationSection of Medical Entomology, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-0425, USA.lld:pubmed
pubmed-article:11986214pubmed:publicationTypeJournal Articlelld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11986214lld:pubmed
More...