| pubmed-article:11969247 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11969247 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:11969247 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:11969247 | lifeskim:mentions | umls-concept:C0229535 | lld:lifeskim |
| pubmed-article:11969247 | lifeskim:mentions | umls-concept:C0032016 | lld:lifeskim |
| pubmed-article:11969247 | lifeskim:mentions | umls-concept:C0178719 | lld:lifeskim |
| pubmed-article:11969247 | lifeskim:mentions | umls-concept:C0234388 | lld:lifeskim |
| pubmed-article:11969247 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:11969247 | lifeskim:mentions | umls-concept:C1707520 | lld:lifeskim |
| pubmed-article:11969247 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:11969247 | pubmed:dateCreated | 2002-4-23 | lld:pubmed |
| pubmed-article:11969247 | pubmed:abstractText | Simultaneous measurements of electrical activity and intracellular Ca(2+) levels were performed in perforated-patch current-clamped individual GH3 cells. Both in cells showing brief (<100 ms) and long action potentials (APs), we found a good correlation between the averaged intracellular Ca2+ concentration ([Ca2+]i) and AP frequency, but not between the mean [Ca2+]i and AP duration. Nevertheless, the magnitude of spontaneous Ca2+ oscillations was highly dependent on the size and duration of the APs. The decay of the Ca2+ transients was not slowed when the size of the oscillations was varied either spontaneously or after elongation of the AP with the K+ channel blocker tetraethyl ammonium. Furthermore, the recovery from Ca2+ loads similar to those induced by the APs was slightly retarded after treatment of the cells with intracellular store Ca2+-ATPase inhibitors. Among previous results showing that caffeine-induced [Ca2+]i increases are secondary to electrical activity enhancements in GH3 cells, these data indicate that the Ca2+ entry triggered via APs is the primary determinant of the [Ca2+]i variations, and that Ca2+-induced Ca2+ release has a minor contribution to Ca2+ oscillations recorded during spontaneous activity. They also point to modulation of electrical activity patterns as a crucial factor regulating spontaneous [Ca2+]i signalling, and hence pituitary cell functions in response to physiological secretagogues. | lld:pubmed |
| pubmed-article:11969247 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11969247 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11969247 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11969247 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11969247 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11969247 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11969247 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11969247 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11969247 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:11969247 | pubmed:issn | 0143-4160 | lld:pubmed |
| pubmed-article:11969247 | pubmed:author | pubmed-author:BarrowBB | lld:pubmed |
| pubmed-article:11969247 | pubmed:author | pubmed-author:de la PeñaPP | lld:pubmed |
| pubmed-article:11969247 | pubmed:author | pubmed-author:GiráldezTT | lld:pubmed |
| pubmed-article:11969247 | pubmed:author | pubmed-author:Gómez-VarelaD... | lld:pubmed |
| pubmed-article:11969247 | pubmed:copyrightInfo | Copyright 2002 Elsevier Science Ltd. All rights reserved. | lld:pubmed |
| pubmed-article:11969247 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11969247 | pubmed:volume | 31 | lld:pubmed |
| pubmed-article:11969247 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11969247 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11969247 | pubmed:pagination | 65-78 | lld:pubmed |
| pubmed-article:11969247 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:11969247 | pubmed:meshHeading | pubmed-meshheading:11969247... | lld:pubmed |
| pubmed-article:11969247 | pubmed:meshHeading | pubmed-meshheading:11969247... | lld:pubmed |
| pubmed-article:11969247 | pubmed:meshHeading | pubmed-meshheading:11969247... | lld:pubmed |
| pubmed-article:11969247 | pubmed:meshHeading | pubmed-meshheading:11969247... | lld:pubmed |
| pubmed-article:11969247 | pubmed:meshHeading | pubmed-meshheading:11969247... | lld:pubmed |
| pubmed-article:11969247 | pubmed:meshHeading | pubmed-meshheading:11969247... | lld:pubmed |
| pubmed-article:11969247 | pubmed:meshHeading | pubmed-meshheading:11969247... | lld:pubmed |
| pubmed-article:11969247 | pubmed:meshHeading | pubmed-meshheading:11969247... | lld:pubmed |
| pubmed-article:11969247 | pubmed:meshHeading | pubmed-meshheading:11969247... | lld:pubmed |
| pubmed-article:11969247 | pubmed:meshHeading | pubmed-meshheading:11969247... | lld:pubmed |
| pubmed-article:11969247 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:11969247 | pubmed:articleTitle | Correlation between electrical activity and intracellular Ca2+ oscillations in GH3 rat anterior pituitary cells. | lld:pubmed |
| pubmed-article:11969247 | pubmed:affiliation | Departamento de Bioquímica y Biología Molecular. Edificio Santiago Gascón, Campus del Cristo, Universidad de Oviedo, Oviedo, Spain. | lld:pubmed |
| pubmed-article:11969247 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11969247 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11969247 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11969247 | lld:pubmed |
