| pubmed-article:11836407 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11836407 | lifeskim:mentions | umls-concept:C0935640 | lld:lifeskim |
| pubmed-article:11836407 | lifeskim:mentions | umls-concept:C0058594 | lld:lifeskim |
| pubmed-article:11836407 | lifeskim:mentions | umls-concept:C0125440 | lld:lifeskim |
| pubmed-article:11836407 | lifeskim:mentions | umls-concept:C0598312 | lld:lifeskim |
| pubmed-article:11836407 | lifeskim:mentions | umls-concept:C1521761 | lld:lifeskim |
| pubmed-article:11836407 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:11836407 | pubmed:dateCreated | 2002-2-11 | lld:pubmed |
| pubmed-article:11836407 | pubmed:abstractText | The baculovirus replication factors LEF-1 and LEF-2 of the Autographa californica multinucleocapsid nucleopolyhedrovirus were overexpressed as fusions containing a hemagglutinin (HA) epitope and a HIS(6) tag using recombinant baculoviruses. LEF-1 was purified to near homogeneity and found to have primase activity in an indirect assay employing Escherichia coli DNA polymerase I (Klenow enzyme) and poly(dT) template. The LEF-1 primase products were also directly characterized by electrophoresis in 20% polyacrylamide-8 M urea gels and agarose gels. Primer synthesis was time dependent, and products of several hundred nucleotides or more were observed from the M13 single-stranded DNA (ssDNA) template. The LEF-1 primase was absolutely dependent on divalent cations (Mg(2+)), and optimal activity was supported by 10 mM MgCl(2). An alkaline pH (8.8 to 9.4) was optimal, whereas monovalent salt (KCl) was inhibitory. Mutation of an invariant aspartic acid in a putative primase domain caused LEF-1 activity to be abolished. Upon ultracentrifugation in glycerol gradients, LEF-1 was found to have a sedimentation coefficient of 3S that is consistent with its being present as a monomer. Elution profiles of LEF-1 and LEF-2 from ssDNA-cellulose and DEAE resin suggested that LEF-2 may bind to both DNA and LEF-1. | lld:pubmed |
| pubmed-article:11836407 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11836407 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11836407 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11836407 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11836407 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:11836407 | pubmed:issn | 0022-538X | lld:pubmed |
| pubmed-article:11836407 | pubmed:author | pubmed-author:MikhailovVict... | lld:pubmed |
| pubmed-article:11836407 | pubmed:author | pubmed-author:RohrmannGeorg... | lld:pubmed |
| pubmed-article:11836407 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11836407 | pubmed:volume | 76 | lld:pubmed |
| pubmed-article:11836407 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11836407 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11836407 | pubmed:pagination | 2287-97 | lld:pubmed |
| pubmed-article:11836407 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:11836407 | pubmed:meshHeading | pubmed-meshheading:11836407... | lld:pubmed |
| pubmed-article:11836407 | pubmed:meshHeading | pubmed-meshheading:11836407... | lld:pubmed |
| pubmed-article:11836407 | pubmed:meshHeading | pubmed-meshheading:11836407... | lld:pubmed |
| pubmed-article:11836407 | pubmed:meshHeading | pubmed-meshheading:11836407... | lld:pubmed |
| pubmed-article:11836407 | pubmed:meshHeading | pubmed-meshheading:11836407... | lld:pubmed |
| pubmed-article:11836407 | pubmed:meshHeading | pubmed-meshheading:11836407... | lld:pubmed |
| pubmed-article:11836407 | pubmed:meshHeading | pubmed-meshheading:11836407... | lld:pubmed |
| pubmed-article:11836407 | pubmed:meshHeading | pubmed-meshheading:11836407... | lld:pubmed |
| pubmed-article:11836407 | pubmed:meshHeading | pubmed-meshheading:11836407... | lld:pubmed |
| pubmed-article:11836407 | pubmed:meshHeading | pubmed-meshheading:11836407... | lld:pubmed |
| pubmed-article:11836407 | pubmed:meshHeading | pubmed-meshheading:11836407... | lld:pubmed |
| pubmed-article:11836407 | pubmed:meshHeading | pubmed-meshheading:11836407... | lld:pubmed |
| pubmed-article:11836407 | pubmed:meshHeading | pubmed-meshheading:11836407... | lld:pubmed |
| pubmed-article:11836407 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:11836407 | pubmed:articleTitle | Baculovirus replication factor LEF-1 is a DNA primase. | lld:pubmed |
| pubmed-article:11836407 | pubmed:affiliation | Department of Microbiology, Oregon State University, Corvallis, OR 97331-3804, USA. vmikhailov@proxima.idb.ac.ru | lld:pubmed |
| pubmed-article:11836407 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11836407 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:11836407 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| family:PF03041.9 | family:pubmed | pubmed-article:11836407 | lld:pfam |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11836407 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11836407 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11836407 | lld:pubmed |
