| pubmed-article:11822454 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11822454 | lifeskim:mentions | umls-concept:C0205250 | lld:lifeskim |
| pubmed-article:11822454 | lifeskim:mentions | umls-concept:C0596258 | lld:lifeskim |
| pubmed-article:11822454 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:11822454 | pubmed:dateCreated | 2002-2-1 | lld:pubmed |
| pubmed-article:11822454 | pubmed:abstractText | Antibody 38C2 efficiently catalyzes deuterium-exchange reactions at the alpha position of a variety of ketones and aldehydes, including substrates that have a variety of sensitive functional groups. In addition to the regio- and chemoselectivity of these reactions, the catalytic rates (kcat) and rate-enhancement values (kcat/kun) are among the highest values ever observed with catalytic antibodies. Comparison of the substrate range of the catalytic antibody with highly evolved aldolase enzymes, such as rabbit-muscle aldolase, highlights the much broader practical scope of the antibody, which accepts a wide range of substrates. The hydrogen-exchange reaction was used for calibration and mapping of the antibody active site. Isotope-exchange experiments with cycloheptanone reveal that the formation of the Schiff base species (as concluded from the 16O/18O exchange rate at the carbonyl oxygen) is much faster than the formation of the enamine intermediate (as concluded from the H/D exchange rate), and both steps are faster than the antibody-catalyzed aldol addition reaction. | lld:pubmed |
| pubmed-article:11822454 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11822454 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11822454 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11822454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11822454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11822454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11822454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11822454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11822454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11822454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11822454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11822454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11822454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11822454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11822454 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11822454 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:11822454 | pubmed:issn | 0947-6539 | lld:pubmed |
| pubmed-article:11822454 | pubmed:author | pubmed-author:ShulmanAvidor... | lld:pubmed |
| pubmed-article:11822454 | pubmed:author | pubmed-author:SitryDanielle... | lld:pubmed |
| pubmed-article:11822454 | pubmed:author | pubmed-author:ShulmanHagitH | lld:pubmed |
| pubmed-article:11822454 | pubmed:author | pubmed-author:KeinanEhudE | lld:pubmed |
| pubmed-article:11822454 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11822454 | pubmed:day | 4 | lld:pubmed |
| pubmed-article:11822454 | pubmed:volume | 8 | lld:pubmed |
| pubmed-article:11822454 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11822454 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11822454 | pubmed:pagination | 229-39 | lld:pubmed |
| pubmed-article:11822454 | pubmed:dateRevised | 2009-8-4 | lld:pubmed |
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| pubmed-article:11822454 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:11822454 | pubmed:articleTitle | Highly efficient antibody-catalyzed deuteration of carbonyl compounds. | lld:pubmed |
| pubmed-article:11822454 | pubmed:affiliation | Department of Chemistry and Institute of Catalysis Science and Technology, Technion-Israel Institute of Technology Technion City, Haifa. | lld:pubmed |
| pubmed-article:11822454 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11822454 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:11822454 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
