| pubmed-article:1174539 | pubmed:abstractText | We hhave been analyzing what could be considered to be structural ribosomal proteins, that is, those that remain with the ribosome when it has been subjected to centrifugation through 1.75 M sucrose, treatment with puromycin and KCl, and centrifugation through 0.5 M KCl and sucrose. By two-dimensional gel electrophoresis, a mouse-specific protein and a Syrian hamster-specific protein are detected, using liver or cultured cell lines. With liquid scintillation counting after two-dimensional gel electrophoresis it is possible to estimate the proportion of species-specific ribosomal protein in a mouse-hamster somatic cell hybrid. | lld:pubmed |
