pubmed-article:11683408 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:11683408 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
pubmed-article:11683408 | lifeskim:mentions | umls-concept:C0346647 | lld:lifeskim |
pubmed-article:11683408 | lifeskim:mentions | umls-concept:C0162638 | lld:lifeskim |
pubmed-article:11683408 | lifeskim:mentions | umls-concept:C0334227 | lld:lifeskim |
pubmed-article:11683408 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
pubmed-article:11683408 | lifeskim:mentions | umls-concept:C0021469 | lld:lifeskim |
pubmed-article:11683408 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
pubmed-article:11683408 | lifeskim:mentions | umls-concept:C0598002 | lld:lifeskim |
pubmed-article:11683408 | pubmed:issue | Pt 15 | lld:pubmed |
pubmed-article:11683408 | pubmed:dateCreated | 2001-10-30 | lld:pubmed |
pubmed-article:11683408 | pubmed:abstractText | In this study we investigated the functional role of FAP-1 as a potential inhibitor of CD95 (Fas, APO-1)-mediated apoptosis in pancreatic cancer cells. Stable transfection of the CD95-sensitive, FAP-1-negative cell line Capan-1 with an FAP-1 cDNA resulted in a strongly decreased sensitivity to CD95-induced apoptosis, as measured by DNA fragmentation and caspase-3 activity. Inhibition of cellular protein tyrosine phosphatases with orthovanadate dose-dependently increased CD95-induced apoptosis in CD95-resistant FAP-1-positive Panc89 and Capan-1-FAP-1 cells almost to the level seen in wild-type Capan-1 cells. Blocking the CD95/FAP-1 interaction in Panc89 cells by cytoplasmic microinjection of a synthetic tripeptide mimicking the C terminus of CD95 resulted in a mean 5.5-fold increase in apoptosis compared to cells that received a control peptide. Using confocal laser scanning microscopy we show that in Panc89 cells FAP-1 is mainly associated with the Golgi complex and with peripheral vesicles. FAP-1 displayed enhanced colocalization with CD95 upon CD95 stimulation in the Golgi complex but not in surface-associated vesicles. This correlated with a decrease in plasma membrane staining for CD95 as determined by FACS analysis. Inhibition of Golgi anterograde transport by brefeldin A abolished the anti-CD95-induced colocalization of FAP-1 and CD95 as well as the decrease in cell-surface-associated CD95. Finally, we demonstrate by immunohistochemistry that FAP-1 is strongly expressed in tumor cells from pancreatic carcinoma tissues. Taken together, these results show that FAP-1 can protect pancreatic carcinoma cells from CD95-mediated apoptosis, probably by preventing anti-CD95-induced translocation of CD95 from intracellular stores to the cell surface. | lld:pubmed |
pubmed-article:11683408 | pubmed:language | eng | lld:pubmed |
pubmed-article:11683408 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:11683408 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:11683408 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:11683408 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:11683408 | pubmed:month | Aug | lld:pubmed |
pubmed-article:11683408 | pubmed:issn | 0021-9533 | lld:pubmed |
pubmed-article:11683408 | pubmed:author | pubmed-author:KalthoffHH | lld:pubmed |
pubmed-article:11683408 | pubmed:author | pubmed-author:RoederCC | lld:pubmed |
pubmed-article:11683408 | pubmed:author | pubmed-author:Henne-BrunsDD | lld:pubmed |
pubmed-article:11683408 | pubmed:author | pubmed-author:UngefrorenHH | lld:pubmed |
pubmed-article:11683408 | pubmed:author | pubmed-author:ArltAA | lld:pubmed |
pubmed-article:11683408 | pubmed:author | pubmed-author:KruseM LML | lld:pubmed |
pubmed-article:11683408 | pubmed:author | pubmed-author:TrauzoldAA | lld:pubmed |
pubmed-article:11683408 | pubmed:author | pubmed-author:RoeschmannSS | lld:pubmed |
pubmed-article:11683408 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:11683408 | pubmed:volume | 114 | lld:pubmed |
pubmed-article:11683408 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:11683408 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:11683408 | pubmed:pagination | 2735-46 | lld:pubmed |
pubmed-article:11683408 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
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pubmed-article:11683408 | pubmed:year | 2001 | lld:pubmed |
pubmed-article:11683408 | pubmed:articleTitle | FAP-1 in pancreatic cancer cells: functional and mechanistic studies on its inhibitory role in CD95-mediated apoptosis. | lld:pubmed |
pubmed-article:11683408 | pubmed:affiliation | Clinic for General Surgery and Thoracic Surgery, Christian-Albrechts-University, Kiel, Germany. | lld:pubmed |
pubmed-article:11683408 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:11683408 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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