| pubmed-article:115899 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:115899 | lifeskim:mentions | umls-concept:C0014441 | lld:lifeskim |
| pubmed-article:115899 | lifeskim:mentions | umls-concept:C0072544 | lld:lifeskim |
| pubmed-article:115899 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:115899 | pubmed:dateCreated | 1980-1-19 | lld:pubmed |
| pubmed-article:115899 | pubmed:abstractText | An enzyme-linked immunosorbent assay (ELISA) is described for Pseudomonas aeruginosa exotoxin A. A double antibody sandwich method was used, employing polyvinyl microtiter plates as the solid phase, a primary coat of monospecific rabbit antitoxin serum, an outer layer composed of a horseradish peroxidase-sheep antitoxin immunoglobulin G conjugate, and an ortho-phenylene-diamine substrate. Absorbance (optical density) of hydrolyzed end product was read spectrophotometrically at 492 nm. ELISA detected as little as 30 pg (0.3 ng/ml) of purified toxin, and absorbance was linear over a 20-fold or greater concentration range. Toxin was demonstrated in culture filtrates from 42 of 48 (88%) consecutive clinical P. aeruginosa isolates compared with 37 of 48 (77%) positive by hemagglutination inhibition. Results of the two assays correlated closely (r = 0.82, P less than 0.001). Specificity was confirmed by neutralizability of ELISA activity with monospecific antitoxin. ELISA was thus a sensitive, specific, and quantifiable technique for the assay of P. aeruginosa exotoxin A in both purified and crude culture materials. | lld:pubmed |
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| pubmed-article:115899 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:115899 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:115899 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:115899 | pubmed:language | eng | lld:pubmed |
| pubmed-article:115899 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:115899 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:115899 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:115899 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:115899 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:115899 | pubmed:issn | 0095-1137 | lld:pubmed |
| pubmed-article:115899 | pubmed:author | pubmed-author:PollackMM | lld:pubmed |
| pubmed-article:115899 | pubmed:author | pubmed-author:SchultzW WWW | lld:pubmed |
| pubmed-article:115899 | pubmed:author | pubmed-author:PhippsT JTJ | lld:pubmed |
| pubmed-article:115899 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:115899 | pubmed:volume | 9 | lld:pubmed |
| pubmed-article:115899 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:115899 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:115899 | pubmed:pagination | 705-8 | lld:pubmed |
| pubmed-article:115899 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:115899 | pubmed:meshHeading | pubmed-meshheading:115899-H... | lld:pubmed |
| pubmed-article:115899 | pubmed:meshHeading | pubmed-meshheading:115899-P... | lld:pubmed |
| pubmed-article:115899 | pubmed:meshHeading | pubmed-meshheading:115899-P... | lld:pubmed |
| pubmed-article:115899 | pubmed:meshHeading | pubmed-meshheading:115899-H... | lld:pubmed |
| pubmed-article:115899 | pubmed:meshHeading | pubmed-meshheading:115899-E... | lld:pubmed |
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| pubmed-article:115899 | pubmed:meshHeading | pubmed-meshheading:115899-E... | lld:pubmed |
| pubmed-article:115899 | pubmed:year | 1979 | lld:pubmed |
| pubmed-article:115899 | pubmed:articleTitle | Enzyme-linked immunosorbent assay for Pseudomonas aeruginosa exotoxin A. | lld:pubmed |
| pubmed-article:115899 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:115899 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:115899 | lld:pubmed |
