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pubmed-article:11563963pubmed:abstractTextThe main properties (pH and temperature range, stability, substrate specificity) of the modified cellulase CelE (endo-beta-1,4-glucanase) from Clostridium thermocellum have been analyzed with the goal of its expression in plants. The modified enzyme is similar to plant cellulases. Deletions in the N-terminus of the enzyme do not affect its biochemical properties. Based on the present investigation, we conclude that the modified beta-1,4-glucanase CelEM1, when expressed in plants, will be a good model to study the role of cellulases in plants.lld:pubmed
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pubmed-article:11563963pubmed:authorpubmed-author:PiruzianE SESlld:pubmed
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pubmed-article:11563963pubmed:dateRevised2010-11-18lld:pubmed
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pubmed-article:11563963pubmed:year2001lld:pubmed
pubmed-article:11563963pubmed:articleTitleExploring the properties of thermostable Clostridium thermocellum cellulase CelE for the purpose of its expression in plants.lld:pubmed
pubmed-article:11563963pubmed:affiliationVavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991, Russia. roustam@vigg.rulld:pubmed
pubmed-article:11563963pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:11563963pubmed:publicationTypeComparative Studylld:pubmed
pubmed-article:11563963pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed