| pubmed-article:1155836 | pubmed:abstractText | From 18 donor dogs of different breeds and ages, follicular lesions of the third eyelid (plica senilunaris conjunctiva) and genitalia were surgically removed, trypsinized, and inoculated on monolayers of HeLa, rabbit kidney, and canine kidney cell cultures. Blind passages of the lesion material were made every 96 hours for 10 to 15 cell culture passages. Cellular suspensions prepared from the lesions were grown in test tubes and passaged 3 times at 10-day intervals between passages. All cultures were observed each day for cytopathic effect. Transmission studies were made by (1) inoculating normal pups with cellular suspensions of the lesions from an infected dog and an infected pup, (2) placing normal pups in contact with infected ones for contact transmission, and (3) inoculating normal animals with cell suspensions prepared from inoculated monolayers. Cytopathic changes were not seen in any of the cell culture monolayers. All transmission attempts were successful, in that characteristic lesions comparable in appearance to those seen in natural infections were produced in susceptible pups. The lesion material from an infected pup was found to be infective for a normal pup after 6 passages in tissue culture (primary rabbit kidney cells) despite absence of cytopathic effect. | lld:pubmed |
