pubmed-article:11402203 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:11402203 | lifeskim:mentions | umls-concept:C0032098 | lld:lifeskim |
pubmed-article:11402203 | lifeskim:mentions | umls-concept:C0162741 | lld:lifeskim |
pubmed-article:11402203 | lifeskim:mentions | umls-concept:C0003069 | lld:lifeskim |
pubmed-article:11402203 | lifeskim:mentions | umls-concept:C0086439 | lld:lifeskim |
pubmed-article:11402203 | lifeskim:mentions | umls-concept:C0007009 | lld:lifeskim |
pubmed-article:11402203 | lifeskim:mentions | umls-concept:C0205349 | lld:lifeskim |
pubmed-article:11402203 | lifeskim:mentions | umls-concept:C0060775 | lld:lifeskim |
pubmed-article:11402203 | pubmed:issue | 2 | lld:pubmed |
pubmed-article:11402203 | pubmed:dateCreated | 2001-6-12 | lld:pubmed |
pubmed-article:11402203 | pubmed:abstractText | The role of fructose-2,6-bisphosphate (Fru-2,6-P(2)) as a regulatory metabolite in photosynthetic carbohydrate metabolism was studied in transgenic Arabidopsis plants with reduced activity of Fru-6-phosphate,2-kinase/Fru-2,6-bisphosphatase. A positive correlation was observed between the Fru-6-phosphate,2-kinase activity and the level of Fru-2,6-P(2) in the leaves. The partitioning of carbon was studied by (14)CO(2) labeling of photosynthetic products. Plant lines with Fru-2,6-P(2) levels down to 5% of the levels observed in wild-type (WT) plants had significantly altered partitioning of carbon between sucrose (Suc) versus starch. The ratio of (14)C incorporated into Suc and starch increased 2- to 3-fold in the plants with low levels of Fru-2,6-P(2) compared with WT. Transgenic plant lines with intermediate levels of Fru-2,6-P(2) compared with WT had a Suc-to-starch labeling ratio similar to the WT. Levels of sugars, starch, and phosphorylated intermediates in leaves were followed during the diurnal cycle. Plants with low levels of Fru-2,6-P(2) in leaves had high levels of Suc, glucose, and Fru and low levels of triose phosphates and glucose-1-P during the light period compared with WT. During the dark period these differences were eliminated. Our data provide direct evidence that Fru-2,6-P(2) affects photosynthetic carbon partitioning in Arabidopsis. Opposed to this, Fru-2,6-P(2) does not contribute significantly to regulation of metabolite levels in darkness. | lld:pubmed |
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pubmed-article:11402203 | pubmed:language | eng | lld:pubmed |
pubmed-article:11402203 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:11402203 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:11402203 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:11402203 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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pubmed-article:11402203 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:11402203 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:11402203 | pubmed:month | Jun | lld:pubmed |
pubmed-article:11402203 | pubmed:issn | 0032-0889 | lld:pubmed |
pubmed-article:11402203 | pubmed:author | pubmed-author:NielsenT HTH | lld:pubmed |
pubmed-article:11402203 | pubmed:author | pubmed-author:DraborgHH | lld:pubmed |
pubmed-article:11402203 | pubmed:author | pubmed-author:VilladsenDD | lld:pubmed |
pubmed-article:11402203 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:11402203 | pubmed:volume | 126 | lld:pubmed |
pubmed-article:11402203 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:11402203 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:11402203 | pubmed:pagination | 750-8 | lld:pubmed |
pubmed-article:11402203 | pubmed:dateRevised | 2010-9-14 | lld:pubmed |
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pubmed-article:11402203 | pubmed:year | 2001 | lld:pubmed |
pubmed-article:11402203 | pubmed:articleTitle | Transgenic Arabidopsis plants with decreased activity of fructose-6-phosphate,2-kinase/fructose-2,6-bisphosphatase have altered carbon partitioning. | lld:pubmed |
pubmed-article:11402203 | pubmed:affiliation | Plant Biochemistry Laboratory, Department of Plant Biology, The Royal Veterinary and Agricultural University, 40 Thorvaldsensvej, DK-1871 Frederiksberg C, Copenhagen, Denmark. | lld:pubmed |
pubmed-article:11402203 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:11402203 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
entrez-gene:837221 | entrezgene:pubmed | pubmed-article:11402203 | lld:entrezgene |
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