| pubmed-article:11375201 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11375201 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:11375201 | lifeskim:mentions | umls-concept:C1007163 | lld:lifeskim |
| pubmed-article:11375201 | lifeskim:mentions | umls-concept:C0005576 | lld:lifeskim |
| pubmed-article:11375201 | lifeskim:mentions | umls-concept:C0950252 | lld:lifeskim |
| pubmed-article:11375201 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:11375201 | pubmed:dateCreated | 2001-5-25 | lld:pubmed |
| pubmed-article:11375201 | pubmed:abstractText | The toluene-degrading strain Rhodococcus opacus PWD4 was found to hydroxylate D-limonene exclusively in the 6-position, yielding enantiomerically pure (+) trans-carveol and traces of (+) carvone. This biotransformation was studied using cells cultivated in chemostat culture with toluene as a carbon and energy source. The maximal specific activity of (+) trans-carveol formation was 14.7 U (g of cells [dry weight])(-1), and the final yield was 94 to 97%. Toluene was found to be a strong competitive inhibitor of the D-limonene conversion. Glucose-grown cells did not form any trans-carveol from D-limonene. These results suggest that one of the enzymes involved in toluene degradation is responsible for this allylic monohydroxylation. Another toluene degrader (Rhodococcus globerulus PWD8) had a lower specific activity but was found to oxidize most of the formed trans-carveol to (+) carvone, allowing for the biocatalytic production of this flavor compound. | lld:pubmed |
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| pubmed-article:11375201 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11375201 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11375201 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:11375201 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11375201 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:11375201 | pubmed:issn | 0099-2240 | lld:pubmed |
| pubmed-article:11375201 | pubmed:author | pubmed-author:ReaFF | lld:pubmed |
| pubmed-article:11375201 | pubmed:author | pubmed-author:WitholtBB | lld:pubmed |
| pubmed-article:11375201 | pubmed:author | pubmed-author:DuetzW AWA | lld:pubmed |
| pubmed-article:11375201 | pubmed:author | pubmed-author:FjällmanA HAH | lld:pubmed |
| pubmed-article:11375201 | pubmed:author | pubmed-author:JourdatCC | lld:pubmed |
| pubmed-article:11375201 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11375201 | pubmed:volume | 67 | lld:pubmed |
| pubmed-article:11375201 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11375201 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11375201 | pubmed:pagination | 2829-32 | lld:pubmed |
| pubmed-article:11375201 | pubmed:dateRevised | 2010-9-14 | lld:pubmed |
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| pubmed-article:11375201 | pubmed:meshHeading | pubmed-meshheading:11375201... | lld:pubmed |
| pubmed-article:11375201 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11375201 | pubmed:articleTitle | Biotransformation of D-limonene to (+) trans-carveol by toluene-grown Rhodococcus opacus PWD4 cells. | lld:pubmed |
| pubmed-article:11375201 | pubmed:affiliation | Institute of Biotechnology, ETH Hönggerberg, HPT, CH 8093, Zürich, Switzerland. duetz@biotech.biol.ethz.ch | lld:pubmed |
| pubmed-article:11375201 | pubmed:publicationType | Journal Article | lld:pubmed |
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