| pubmed-article:11316763 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11316763 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:11316763 | lifeskim:mentions | umls-concept:C0030520 | lld:lifeskim |
| pubmed-article:11316763 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:11316763 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:11316763 | lifeskim:mentions | umls-concept:C1515655 | lld:lifeskim |
| pubmed-article:11316763 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:11316763 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:11316763 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:11316763 | pubmed:dateCreated | 2001-4-24 | lld:pubmed |
| pubmed-article:11316763 | pubmed:abstractText | PTH and PTH-related protein (PTHrP) are key mediators of skeletal development and homeostasis through their activation of the PTH-1 receptor. Previous studies have found that several AP-1 family members are regulated by PTH, such as c-fos, fra-1, and c-jun. There are numerous genes in the bone microenvironment that contain AP-1 sites, and different Fos family members are reported to have opposing transcriptional activities at AP-1 sites. The purpose of this study was to identify the effects of PTH on expression of the AP-1 protein complex member, fra-2, to extend our understanding of transcriptional regulators of PTH action. PTH induction of fra-2 messenger RNA (mRNA) levels in MC3T3-E1 preosteoblastic cells was maximal with 0.1 microM PTH (1-34). The expression in vitro was greatest 1 h after treatment and was present with N-terminal PTH but not PTH (7-34) or (53-84). Cycloheximide treatment induced fra-2 expression, and actinomycin D inhibited basal and PTHrP-induced expression. AP-1 protein in nuclear extracts of MC3T3-E1 cells was increased with PTH treatment at 3 h and consisted of high levels of Fra-2 protein, as evidenced by a supershift in an electrophoretic mobility shift assay and Western blot analysis. Up-regulation of steady-state fra-2 mRNA was also noted in vivo, where injection of PTH (1-34) (20 microgram) resulted in a more-than-7-fold maximal increase in fra-2 mRNA expression in the calvaria of mice, after 1 h of treatment. These data add to the transcriptional mediators induced by PTH and suggest that the interplay of AP-1 family members will provide insight into regulatory pathways of PTH and PTHrP for their anabolic and catabolic actions in bone. | lld:pubmed |
| pubmed-article:11316763 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11316763 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11316763 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11316763 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:11316763 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11316763 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11316763 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:11316763 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11316763 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11316763 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11316763 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11316763 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11316763 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11316763 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11316763 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11316763 | pubmed:month | May | lld:pubmed |
| pubmed-article:11316763 | pubmed:issn | 0013-7227 | lld:pubmed |
| pubmed-article:11316763 | pubmed:author | pubmed-author:ChenCC | lld:pubmed |
| pubmed-article:11316763 | pubmed:author | pubmed-author:ChenHH | lld:pubmed |
| pubmed-article:11316763 | pubmed:author | pubmed-author:IrwinRR | lld:pubmed |
| pubmed-article:11316763 | pubmed:author | pubmed-author:OntiverosCC | lld:pubmed |
| pubmed-article:11316763 | pubmed:author | pubmed-author:McCauleyL KLK | lld:pubmed |
| pubmed-article:11316763 | pubmed:author | pubmed-author:McCabeL RLR | lld:pubmed |
| pubmed-article:11316763 | pubmed:author | pubmed-author:Koh-PaigeA... | lld:pubmed |
| pubmed-article:11316763 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11316763 | pubmed:volume | 142 | lld:pubmed |
| pubmed-article:11316763 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11316763 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11316763 | pubmed:pagination | 1975-81 | lld:pubmed |
| pubmed-article:11316763 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:11316763 | pubmed:meshHeading | pubmed-meshheading:11316763... | lld:pubmed |
| pubmed-article:11316763 | pubmed:meshHeading | pubmed-meshheading:11316763... | lld:pubmed |
| pubmed-article:11316763 | pubmed:meshHeading | pubmed-meshheading:11316763... | lld:pubmed |
| pubmed-article:11316763 | pubmed:meshHeading | pubmed-meshheading:11316763... | lld:pubmed |
| pubmed-article:11316763 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11316763 | pubmed:articleTitle | Parathyroid hormone stimulates fra-2 expression in osteoblastic cells in vitro and in vivo. | lld:pubmed |
| pubmed-article:11316763 | pubmed:affiliation | Department of Periodontics/Prevention/Geriatrics, University of Michigan, Ann Arbor, Michigan 48109-1078, USA. | lld:pubmed |
| pubmed-article:11316763 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11316763 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:11316763 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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