| pubmed-article:11292590 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11292590 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:11292590 | lifeskim:mentions | umls-concept:C0242275 | lld:lifeskim |
| pubmed-article:11292590 | lifeskim:mentions | umls-concept:C0021853 | lld:lifeskim |
| pubmed-article:11292590 | lifeskim:mentions | umls-concept:C0026046 | lld:lifeskim |
| pubmed-article:11292590 | lifeskim:mentions | umls-concept:C0033634 | lld:lifeskim |
| pubmed-article:11292590 | lifeskim:mentions | umls-concept:C0085403 | lld:lifeskim |
| pubmed-article:11292590 | lifeskim:mentions | umls-concept:C1545588 | lld:lifeskim |
| pubmed-article:11292590 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:11292590 | lifeskim:mentions | umls-concept:C1554080 | lld:lifeskim |
| pubmed-article:11292590 | lifeskim:mentions | umls-concept:C1706198 | lld:lifeskim |
| pubmed-article:11292590 | lifeskim:mentions | umls-concept:C0173022 | lld:lifeskim |
| pubmed-article:11292590 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:11292590 | pubmed:dateCreated | 2001-4-9 | lld:pubmed |
| pubmed-article:11292590 | pubmed:abstractText | Using monolayers of human intestinal (Caco-2) cells, we showed that growth factors (GFs) protect microtubules and barrier integrity against oxidative injury. Studies in nongastrointestinal cell models suggest that protein kinase C (PKC) signaling is key in GF-induced effects and that cytosolic calcium concentration ([Ca2+](i)) is essential in cell integrity. We hypothesized that GF protection involves activating PKC and maintaining normal ([Ca2+](i)) Monolayers were pretreated with epidermal growth factor (EGF) or PKC or Ca2+ modulators before exposure to oxidants (H2O2 or HOCl). Oxidants disrupted microtubules and barrier integrity, and EGF protected from this damage. EGF caused rapid distribution of PKC-alpha, PKC-betaI, and PKC-zeta isoforms to cell membranes, enhancing PKC activity of membrane fractions while reducing PKC activity of cytosolic fractions. EGF enhanced (45)Ca2+ efflux and prevented oxidant-induced (sustained) rises in ([Ca2+](i)). PKC inhibitors abolished and PKC activators mimicked EGF protection. Oxidant damage was mimicked by and potentiated by a Ca2+ ionophore (A-23187), exacerbated by high-Ca2+ media, and prevented by calcium removal or chelation or by Ca2+ channel antagonists. PKC activators mimicked EGF on both (45)Ca2+ efflux and ([Ca2+](i)). Membrane Ca2+-ATPase pump inhibitors prevented protection by EGF or PKC activators. In conclusion, EGF protection of microtubules and the intestinal epithelial barrier requires activation of PKC signal transduction and normalization of ([Ca2+](i)). | lld:pubmed |
| pubmed-article:11292590 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11292590 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11292590 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11292590 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11292590 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11292590 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11292590 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11292590 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11292590 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11292590 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11292590 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11292590 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11292590 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11292590 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11292590 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11292590 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11292590 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11292590 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11292590 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11292590 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11292590 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11292590 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11292590 | pubmed:month | May | lld:pubmed |
| pubmed-article:11292590 | pubmed:issn | 0193-1857 | lld:pubmed |
| pubmed-article:11292590 | pubmed:author | pubmed-author:FieldsJ ZJZ | lld:pubmed |
| pubmed-article:11292590 | pubmed:author | pubmed-author:BanakDD | lld:pubmed |
| pubmed-article:11292590 | pubmed:author | pubmed-author:ZhangYY | lld:pubmed |
| pubmed-article:11292590 | pubmed:author | pubmed-author:KeshavarzianA... | lld:pubmed |
| pubmed-article:11292590 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11292590 | pubmed:volume | 280 | lld:pubmed |
| pubmed-article:11292590 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11292590 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11292590 | pubmed:pagination | G828-43 | lld:pubmed |
| pubmed-article:11292590 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:11292590 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11292590 | pubmed:articleTitle | Key role of PKC and Ca2+ in EGF protection of microtubules and intestinal barrier against oxidants. | lld:pubmed |
| pubmed-article:11292590 | pubmed:affiliation | Department of Internal Medicine (Division of Digestive Diseases), Rush University Medical Center, 1725 W. Harrison, Suite 206, Chicago, IL 60612, USA. ali_banan@rush.edu | lld:pubmed |
| pubmed-article:11292590 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11292590 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
