| pubmed-article:11289158 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11289158 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:11289158 | lifeskim:mentions | umls-concept:C0014597 | lld:lifeskim |
| pubmed-article:11289158 | lifeskim:mentions | umls-concept:C0105770 | lld:lifeskim |
| pubmed-article:11289158 | lifeskim:mentions | umls-concept:C0334227 | lld:lifeskim |
| pubmed-article:11289158 | lifeskim:mentions | umls-concept:C0085304 | lld:lifeskim |
| pubmed-article:11289158 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:11289158 | lifeskim:mentions | umls-concept:C1566664 | lld:lifeskim |
| pubmed-article:11289158 | lifeskim:mentions | umls-concept:C1527249 | lld:lifeskim |
| pubmed-article:11289158 | lifeskim:mentions | umls-concept:C0040624 | lld:lifeskim |
| pubmed-article:11289158 | lifeskim:mentions | umls-concept:C0301625 | lld:lifeskim |
| pubmed-article:11289158 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:11289158 | pubmed:dateCreated | 2001-4-5 | lld:pubmed |
| pubmed-article:11289158 | pubmed:abstractText | Beta-catenin acts as a transcriptional coactivator by forming a complex with T-cell factor/lymphoid enhancer factor (TCF/LEF) DNA-binding proteins. Aberrant transactivation of a certain set of target genes by beta-catenin and TCF4 complexes has been implicated in familial and sporadic colorectal tumorigenesis. A colorectal cancer cell line, DLD-1, becomes irregularly multilayered, when maintained confluent for 2-3 weeks, and forms numerous dome-like polypoid foci piled-up over the surface of cell sheets. By the use of a strict tetracycline-regulation system, we found that the continuous suppression of beta-catenin/TCF4-mediated gene transactivation by dominant-negative TCF4B (deltaN30) reduced these piled-up foci and restored a simple monolayer of polarized columnar cells resembling normal intestinal epithelium. The restoration of epithelial cell polarity was evident in two ways: (a) the formation of microvilli over the apical surface; and (b) the distribution of a tight junction protein, ZO-1, to the lateral plasma membrane. Retroviral expression of stabilized beta-catenin (deltaN89) induced the formation of similar piled-up foci in untransformed IEC6 intestinal epithelial cells. Sulindac, a nonsteroidal antiinflammatory drug effective against colorectal tumorigenesis in familial adenomatous polyposis syndrome, suppressed the formation of foci. The loss of epithelial cell polarity may be a critical cellular event driving beta-catenin/TCF4-mediated intestinal tumorigenesis. | lld:pubmed |
| pubmed-article:11289158 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11289158 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11289158 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:11289158 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11289158 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11289158 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:11289158 | pubmed:issn | 0008-5472 | lld:pubmed |
| pubmed-article:11289158 | pubmed:author | pubmed-author:ImaiKK | lld:pubmed |
| pubmed-article:11289158 | pubmed:author | pubmed-author:YamadaTT | lld:pubmed |
| pubmed-article:11289158 | pubmed:author | pubmed-author:HayashiRR | lld:pubmed |
| pubmed-article:11289158 | pubmed:author | pubmed-author:HirohashiSS | lld:pubmed |
| pubmed-article:11289158 | pubmed:author | pubmed-author:HasegawaFF | lld:pubmed |
| pubmed-article:11289158 | pubmed:author | pubmed-author:TakaokaA SAS | lld:pubmed |
| pubmed-article:11289158 | pubmed:author | pubmed-author:NaishiroYY | lld:pubmed |
| pubmed-article:11289158 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11289158 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:11289158 | pubmed:volume | 61 | lld:pubmed |
| pubmed-article:11289158 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11289158 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11289158 | pubmed:pagination | 2751-8 | lld:pubmed |
| pubmed-article:11289158 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:11289158 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11289158 | pubmed:articleTitle | Restoration of epithelial cell polarity in a colorectal cancer cell line by suppression of beta-catenin/T-cell factor 4-mediated gene transactivation. | lld:pubmed |
| pubmed-article:11289158 | pubmed:affiliation | Pathology Division, National Cancer Center Research Institute, Tokyo, Japan. | lld:pubmed |
| pubmed-article:11289158 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11289158 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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