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pubmed-article:11286338pubmed:abstractTextThe sensitivity of optical biosensors where the detection takes place on a planar gold surface can be improved by making the surface porous. The porosity allows a larger number of ligands per surface area resulting in larger optical shifts when interacting with specifically binding analyte molecules. The porous gold was deposited as a thin layer on a planar gold surface by electrochemical deposition in a solution of tetrachloroaurate and lead acetate. A protein, streptavidin, was adsorbed into the formed porous layer and the time course of the adsorption was monitored by in-situ ellipsometry. When the porous layer was 500 nm in thickness a six-fold increase of the ellipsometric response was obtained compared with a planar gold surface. The dependency of porosity and layer thickness was explained with a mathematical model of the gold/porous gold/protein/solution system.lld:pubmed
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pubmed-article:11286338pubmed:authorpubmed-author:MandeniusC...lld:pubmed
pubmed-article:11286338pubmed:authorpubmed-author:van NoortDDlld:pubmed
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pubmed-article:11286338pubmed:volume15lld:pubmed
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pubmed-article:11286338pubmed:dateRevised2009-7-14lld:pubmed
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pubmed-article:11286338pubmed:year2000lld:pubmed
pubmed-article:11286338pubmed:articleTitlePorous gold surfaces for biosensor applications.lld:pubmed
pubmed-article:11286338pubmed:affiliationDepartment of Physics and Measurement Technology, Linköping University, Sweden.lld:pubmed
pubmed-article:11286338pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:11286338pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed