| pubmed-article:11280805 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11280805 | lifeskim:mentions | umls-concept:C0949486 | lld:lifeskim |
| pubmed-article:11280805 | lifeskim:mentions | umls-concept:C1709130 | lld:lifeskim |
| pubmed-article:11280805 | lifeskim:mentions | umls-concept:C0678222 | lld:lifeskim |
| pubmed-article:11280805 | lifeskim:mentions | umls-concept:C1510470 | lld:lifeskim |
| pubmed-article:11280805 | lifeskim:mentions | umls-concept:C0812204 | lld:lifeskim |
| pubmed-article:11280805 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:11280805 | lifeskim:mentions | umls-concept:C0392756 | lld:lifeskim |
| pubmed-article:11280805 | lifeskim:mentions | umls-concept:C1511679 | lld:lifeskim |
| pubmed-article:11280805 | lifeskim:mentions | umls-concept:C0702240 | lld:lifeskim |
| pubmed-article:11280805 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:11280805 | pubmed:dateCreated | 2001-3-30 | lld:pubmed |
| pubmed-article:11280805 | pubmed:abstractText | We hypothesize that elevation of Nm23-H1 expression in micrometastatic breast cancer cells may inhibit their metastatic colonization and further invasion, and induce differentiation, thus resulting in a clinical benefit. The current study investigated the possible contribution of DNA methylation to the regulation of Nm23-H1 expression, based on the observation that two CpG islands are present in its promoter. 5-Aza-2'-deoxycytidine (5-Aza-CdR), a DNA methylation inhibitor, increased the Nm23-H1 expression of 5 of 11 human breast carcinoma cell lines in vitro, including 3 of 3 metastatically competent lines. Increased Nm23-H1 expression was accompanied by a reduction in motility in vitro, with minimal effect on proliferation. Both increased Nm23-H1 expression and decreased motility were observed using low (75 nM) concentrations of 5-Aza-CdR. Array analysis of MDA-MB-231 breast carcinoma cells treated with 5-Aza-CdR confirmed the elevation of nm23-H1 mRNA, whereas relatively few other genes exhibited altered expression. Bisulfite sequencing of the two CpG islands in a panel of cell lines and in 20 infiltrating ductal carcinomas revealed that one island (-3090 bp to -3922 bp) exhibited infrequent differential methylation. The data indicate that DNA methylation inhibitors can directly or indirectly cause both elevation of Nm23-H1 expression and decreased function in one aspect of metastasis, motility. | lld:pubmed |
| pubmed-article:11280805 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11280805 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11280805 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11280805 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11280805 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11280805 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11280805 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11280805 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11280805 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11280805 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11280805 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11280805 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11280805 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:11280805 | pubmed:issn | 0008-5472 | lld:pubmed |
| pubmed-article:11280805 | pubmed:author | pubmed-author:OsborneC KCK | lld:pubmed |
| pubmed-article:11280805 | pubmed:author | pubmed-author:ClarkGG | lld:pubmed |
| pubmed-article:11280805 | pubmed:author | pubmed-author:MairMM | lld:pubmed |
| pubmed-article:11280805 | pubmed:author | pubmed-author:SteegP SPS | lld:pubmed |
| pubmed-article:11280805 | pubmed:author | pubmed-author:SgroiDD | lld:pubmed |
| pubmed-article:11280805 | pubmed:author | pubmed-author:ClareS ESE | lld:pubmed |
| pubmed-article:11280805 | pubmed:author | pubmed-author:HartsoughM... | lld:pubmed |
| pubmed-article:11280805 | pubmed:author | pubmed-author:ElkahlounA... | lld:pubmed |
| pubmed-article:11280805 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11280805 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:11280805 | pubmed:volume | 61 | lld:pubmed |
| pubmed-article:11280805 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11280805 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11280805 | pubmed:pagination | 2320-7 | lld:pubmed |
| pubmed-article:11280805 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:11280805 | pubmed:meshHeading | pubmed-meshheading:11280805... | lld:pubmed |
| pubmed-article:11280805 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11280805 | pubmed:articleTitle | Elevation of breast carcinoma Nm23-H1 metastasis suppressor gene expression and reduced motility by DNA methylation inhibition. | lld:pubmed |
| pubmed-article:11280805 | pubmed:affiliation | Division of Clinical Sciences, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA. hartso@box-h.nih.gov | lld:pubmed |
| pubmed-article:11280805 | pubmed:publicationType | Journal Article | lld:pubmed |
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