| pubmed-article:11259493 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11259493 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:11259493 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:11259493 | lifeskim:mentions | umls-concept:C0007603 | lld:lifeskim |
| pubmed-article:11259493 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:11259493 | lifeskim:mentions | umls-concept:C0815000 | lld:lifeskim |
| pubmed-article:11259493 | lifeskim:mentions | umls-concept:C0001473 | lld:lifeskim |
| pubmed-article:11259493 | lifeskim:mentions | umls-concept:C0041904 | lld:lifeskim |
| pubmed-article:11259493 | lifeskim:mentions | umls-concept:C0442805 | lld:lifeskim |
| pubmed-article:11259493 | lifeskim:mentions | umls-concept:C0162493 | lld:lifeskim |
| pubmed-article:11259493 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:11259493 | lifeskim:mentions | umls-concept:C0521115 | lld:lifeskim |
| pubmed-article:11259493 | lifeskim:mentions | umls-concept:C1511938 | lld:lifeskim |
| pubmed-article:11259493 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:11259493 | pubmed:dateCreated | 2001-3-22 | lld:pubmed |
| pubmed-article:11259493 | pubmed:abstractText | Precise regulation of intracellular Ca(2+) concentration ([Ca(2+)](i)) is achieved by the coordinated function of Ca(2+) channels and Ca(2+) buffers. Neuronal differentiation induces up-regulation of Ca(2+) channels. However, little is known about the effects of differentiation on the expression of the plasma membrane Ca(2+)-ATPase (PMCA), the principal Ca(2+) extrusion mechanism in neurons. In this study, we examined the regulation of PMCA expression during differentiation of the human neuroblastoma cell line IMR-32. [Ca(2+)](i) was monitored in single cells using indo-1 microfluorimetry. When the Ca(2+)-ATPase of the endoplasmic reticulum was blocked by cyclopiazonic acid, [Ca(2+)](i) recovery after small depolarization-induced Ca(2+) loads was governed primarily by PMCAs. [Ca(2+)](i) returned to baseline by a process described by a monoexponential function in undifferentiated cells (tau = 52 +/- 4 s; n = 25). After differentiation for 12-16 days, the [Ca(2+)](i) recovery rate increased by more than threefold (tau = 17 +/- 1 s; n = 31). Western blots showed a pronounced increase in expression of three major PMCA isoforms in IMR-32 cells during differentiation, including PMCA2, PMCA3 and PMCA4. These results demonstrate up-regulation of PMCAs on the functional and protein level during neuronal differentiation in vitro. Parallel amplification of Ca(2+) influx and efflux pathways may enable differentiated neurons to precisely localize Ca(2+) signals in time and space. | lld:pubmed |
| pubmed-article:11259493 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259493 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259493 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:11259493 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259493 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11259493 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259493 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11259493 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259493 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:11259493 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259493 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:11259493 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11259493 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:11259493 | pubmed:issn | 0022-3042 | lld:pubmed |
| pubmed-article:11259493 | pubmed:author | pubmed-author:StrehlerE EEE | lld:pubmed |
| pubmed-article:11259493 | pubmed:author | pubmed-author:ThayerS ASA | lld:pubmed |
| pubmed-article:11259493 | pubmed:author | pubmed-author:ToutenhoofdS... | lld:pubmed |
| pubmed-article:11259493 | pubmed:author | pubmed-author:UsachevY MYM | lld:pubmed |
| pubmed-article:11259493 | pubmed:author | pubmed-author:GoellnerG MGM | lld:pubmed |
| pubmed-article:11259493 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11259493 | pubmed:volume | 76 | lld:pubmed |
| pubmed-article:11259493 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11259493 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11259493 | pubmed:pagination | 1756-65 | lld:pubmed |
| pubmed-article:11259493 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:11259493 | pubmed:meshHeading | pubmed-meshheading:11259493... | lld:pubmed |
| pubmed-article:11259493 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11259493 | pubmed:articleTitle | Differentiation induces up-regulation of plasma membrane Ca(2+)-ATPase and concomitant increase in Ca(2+) efflux in human neuroblastoma cell line IMR-32. | lld:pubmed |
| pubmed-article:11259493 | pubmed:affiliation | Department of Pharmacology, University of Minnesota Medical School, Minneapolis 55455, USA. usach001@tc.umn.edu | lld:pubmed |
| pubmed-article:11259493 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11259493 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:11259493 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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