| pubmed-article:11259302 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11259302 | lifeskim:mentions | umls-concept:C1267092 | lld:lifeskim |
| pubmed-article:11259302 | lifeskim:mentions | umls-concept:C0027096 | lld:lifeskim |
| pubmed-article:11259302 | lifeskim:mentions | umls-concept:C0597198 | lld:lifeskim |
| pubmed-article:11259302 | lifeskim:mentions | umls-concept:C1516692 | lld:lifeskim |
| pubmed-article:11259302 | lifeskim:mentions | umls-concept:C0443254 | lld:lifeskim |
| pubmed-article:11259302 | lifeskim:mentions | umls-concept:C1546857 | lld:lifeskim |
| pubmed-article:11259302 | lifeskim:mentions | umls-concept:C2698651 | lld:lifeskim |
| pubmed-article:11259302 | lifeskim:mentions | umls-concept:C1556066 | lld:lifeskim |
| pubmed-article:11259302 | lifeskim:mentions | umls-concept:C1619636 | lld:lifeskim |
| pubmed-article:11259302 | lifeskim:mentions | umls-concept:C1514873 | lld:lifeskim |
| pubmed-article:11259302 | lifeskim:mentions | umls-concept:C0205144 | lld:lifeskim |
| pubmed-article:11259302 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:11259302 | pubmed:dateCreated | 2001-3-22 | lld:pubmed |
| pubmed-article:11259302 | pubmed:abstractText | Myosin II has two heads that are joined together by an alpha-helical coiled-coil rod, which can separate in the region adjacent to the head-rod junction (Trybus, K. M. 1994. J. Biol. Chem. 269:20819-20822). To test whether this flexibility at the head-rod junction is important for the mechanical performance of myosin, we used the optical trap to measure the unitary displacements of heavy meromyosin constructs in which a stable coiled-coil sequence derived from the leucine zipper was introduced into the myosin rod. The zipper was positioned either immediately after the heads (0-hep zip) or following 15 heptads of native sequence (15-hep zip). The unitary displacement (d) decreased from d = 9.7 +/- 0.6 nm for wild-type heavy meromyosin (WT HMM) to d = 0.1 +/- 0.3 nm for the 0-hep zip construct (mean +/- SE). Native values were restored in the 15-hep zip construct (d = 7.5 +/- 0.7 nm). We conclude that flexibility at the myosin head-rod junction, which is provided by an unstable coiled-coil region, is essential for optimal mechanical performance. | lld:pubmed |
| pubmed-article:11259302 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259302 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259302 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259302 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259302 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259302 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259302 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259302 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259302 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259302 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259302 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259302 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259302 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259302 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259302 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259302 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259302 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259302 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259302 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259302 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259302 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259302 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259302 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259302 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259302 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11259302 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259302 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11259302 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259302 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11259302 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:11259302 | pubmed:issn | 0006-3495 | lld:pubmed |
| pubmed-article:11259302 | pubmed:author | pubmed-author:WarshawD MDM | lld:pubmed |
| pubmed-article:11259302 | pubmed:author | pubmed-author:TrybusK MKM | lld:pubmed |
| pubmed-article:11259302 | pubmed:author | pubmed-author:LauzonA MAM | lld:pubmed |
| pubmed-article:11259302 | pubmed:author | pubmed-author:FagnantP MPM | lld:pubmed |
| pubmed-article:11259302 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11259302 | pubmed:volume | 80 | lld:pubmed |
| pubmed-article:11259302 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11259302 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11259302 | pubmed:pagination | 1900-4 | lld:pubmed |
| pubmed-article:11259302 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:11259302 | pubmed:meshHeading | pubmed-meshheading:11259302... | lld:pubmed |
| pubmed-article:11259302 | pubmed:meshHeading | pubmed-meshheading:11259302... | lld:pubmed |
| pubmed-article:11259302 | pubmed:meshHeading | pubmed-meshheading:11259302... | lld:pubmed |
| pubmed-article:11259302 | pubmed:meshHeading | pubmed-meshheading:11259302... | lld:pubmed |
| pubmed-article:11259302 | pubmed:meshHeading | pubmed-meshheading:11259302... | lld:pubmed |
| pubmed-article:11259302 | pubmed:meshHeading | pubmed-meshheading:11259302... | lld:pubmed |
| pubmed-article:11259302 | pubmed:meshHeading | pubmed-meshheading:11259302... | lld:pubmed |
| pubmed-article:11259302 | pubmed:meshHeading | pubmed-meshheading:11259302... | lld:pubmed |
| pubmed-article:11259302 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11259302 | pubmed:articleTitle | Coiled-coil unwinding at the smooth muscle myosin head-rod junction is required for optimal mechanical performance. | lld:pubmed |
| pubmed-article:11259302 | pubmed:affiliation | Meakins-Christie Laboratories, McGill University Health Center, Montreal, Quebec H2X 2P2, Canada. | lld:pubmed |
| pubmed-article:11259302 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11259302 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:11259302 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11259302 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11259302 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11259302 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11259302 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11259302 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11259302 | lld:pubmed |
