| pubmed-article:11259291 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11259291 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:11259291 | lifeskim:mentions | umls-concept:C0401925 | lld:lifeskim |
| pubmed-article:11259291 | lifeskim:mentions | umls-concept:C0026018 | lld:lifeskim |
| pubmed-article:11259291 | lifeskim:mentions | umls-concept:C0014597 | lld:lifeskim |
| pubmed-article:11259291 | lifeskim:mentions | umls-concept:C0043047 | lld:lifeskim |
| pubmed-article:11259291 | lifeskim:mentions | umls-concept:C0022646 | lld:lifeskim |
| pubmed-article:11259291 | lifeskim:mentions | umls-concept:C0680730 | lld:lifeskim |
| pubmed-article:11259291 | lifeskim:mentions | umls-concept:C0022702 | lld:lifeskim |
| pubmed-article:11259291 | lifeskim:mentions | umls-concept:C0031164 | lld:lifeskim |
| pubmed-article:11259291 | lifeskim:mentions | umls-concept:C0444706 | lld:lifeskim |
| pubmed-article:11259291 | lifeskim:mentions | umls-concept:C0392747 | lld:lifeskim |
| pubmed-article:11259291 | lifeskim:mentions | umls-concept:C0596292 | lld:lifeskim |
| pubmed-article:11259291 | lifeskim:mentions | umls-concept:C0023089 | lld:lifeskim |
| pubmed-article:11259291 | lifeskim:mentions | umls-concept:C0441633 | lld:lifeskim |
| pubmed-article:11259291 | lifeskim:mentions | umls-concept:C1148554 | lld:lifeskim |
| pubmed-article:11259291 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:11259291 | pubmed:dateCreated | 2001-3-22 | lld:pubmed |
| pubmed-article:11259291 | pubmed:abstractText | The water channel aquaporin-2 (AQP2), a key component of the antidiuretic machinery in the kidney, is rapidly regulated by the antidiuretic hormone vasopressin. The hormone exerts its action by inducing a translocation of AQP2 from intracellular vesicles to the cell membrane. This step requires the elevation of intracellular cyclic AMP. We describe here a new method, laser scanning reflection microscopy (LSRM), suitable for determining cellular osmotic water permeability coefficient changes in primary cultured inner medullary collecting duct (IMCD) cells. The recording of vertical-reflection-mode x-z-scan section areas of unstained, living IMCD cells proved useful and valid for the investigation of osmotic water permeability changes. The time-dependent increases of reflection-mode x-z-scan section areas of swelling cells were fitted to a single-exponential equation. The analysis of the time constants of these processes indicates a twofold increase in osmotic water permeability of IMCD cells after treatment of the cells both with forskolin, a cyclic AMP-elevating agent, and with Clostridium difficile toxin B, an inhibitor of Rho proteins that leads to depolymerization of F-actin-containing stress fibers. This indicates that both agents lead to the functional insertion of AQP2 into the cell membrane. Thus, we have established a new functional assay for the study of the regulation of the water permeability at the cellular level. | lld:pubmed |
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| pubmed-article:11259291 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11259291 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11259291 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11259291 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:11259291 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11259291 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:11259291 | pubmed:issn | 0006-3495 | lld:pubmed |
| pubmed-article:11259291 | pubmed:author | pubmed-author:BeugHH | lld:pubmed |
| pubmed-article:11259291 | pubmed:author | pubmed-author:LorenzDD | lld:pubmed |
| pubmed-article:11259291 | pubmed:author | pubmed-author:RosenthalWW | lld:pubmed |
| pubmed-article:11259291 | pubmed:author | pubmed-author:WiesnerBB | lld:pubmed |
| pubmed-article:11259291 | pubmed:author | pubmed-author:KlussmannEE | lld:pubmed |
| pubmed-article:11259291 | pubmed:author | pubmed-author:MarieII | lld:pubmed |
| pubmed-article:11259291 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11259291 | pubmed:volume | 80 | lld:pubmed |
| pubmed-article:11259291 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11259291 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11259291 | pubmed:pagination | 1783-90 | lld:pubmed |
| pubmed-article:11259291 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:11259291 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11259291 | pubmed:articleTitle | Cell volume kinetics of adherent epithelial cells measured by laser scanning reflection microscopy: determination of water permeability changes of renal principal cells. | lld:pubmed |
| pubmed-article:11259291 | pubmed:affiliation | Forschungsinstitut für Molekulare Pharmakologie, D-10315 Berlin, Germany. maric@fmp-berlin.de | lld:pubmed |
| pubmed-article:11259291 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11259291 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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