| pubmed-article:11162874 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11162874 | lifeskim:mentions | umls-concept:C0003402 | lld:lifeskim |
| pubmed-article:11162874 | lifeskim:mentions | umls-concept:C0242598 | lld:lifeskim |
| pubmed-article:11162874 | lifeskim:mentions | umls-concept:C0600688 | lld:lifeskim |
| pubmed-article:11162874 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:11162874 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:11162874 | pubmed:dateCreated | 2001-2-22 | lld:pubmed |
| pubmed-article:11162874 | pubmed:abstractText | The present studies were designed to investigate the susceptibility of LLC-PK1 cells to cytotoxicity induced by para-aminophenol (PAP) and the ability of antioxidants to prevent PAP-induced cytotoxicity. LLC-PK1 cells were incubated for 4 h with varying concentrations of PAP (0-0.2 mM). Incubation was continued for 20 h and viability was monitored at 24 h after initial exposure to PAP. For coincubation experiments, cells were incubated for 4 h with various antioxidants [including ascorbate, glutathione (GSH), butylated hydroxytoluene (BHT), beta-nicotinamide adenine dinucleotide (NADH), or beta-nicotinamide adenine dinucleotide phosphate (NADPH)] in the absence or presence of 0.1 mM PAP. For preincubation experiments, cells were incubated for 1 h with ascorbate, GSH or NADPH. Antioxidants were removed and cells were exposed to 0 or 0.1 mM PAP for 4 h. Viability was determined 24 h following PAP exposure. LLC-PK1 cells displayed a steep concentration-response relationship for PAP; 0.1 mM PAP caused approximately 50% loss of viability. Coincubation with ascorbate, GSH and NADPH was without effect on cell viability in the absence of PAP and attenuated PAP-induced losses in viability. In contrast, NADH was ineffective in preventing PAP-induced cytotoxicity. BHT alone produced a significant loss of cell viability and was ineffective in preventing PAP cytotoxicity. Inability of NADH to prevent PAP-induced cytotoxicity was related to rapid degradation of NADH in aqueous solution. Preincubation of cells with ascorbate or GSH but not NADPH was associated with attenuation of PAP-induced cytotoxicity. These data suggest that (1) PAP is cytotoxic to LLC-PK1 cells, (2) a portion of PAP cytotoxicity is due to nonenzymatic oxidation that occurs in the incubation medium, and (3) a portion of PAP cytotoxicity is due to enzymatic or nonenzymatic oxidation that occurs within cells. | lld:pubmed |
| pubmed-article:11162874 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11162874 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11162874 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11162874 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11162874 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11162874 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11162874 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11162874 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11162874 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11162874 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11162874 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11162874 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11162874 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:11162874 | pubmed:issn | 0300-483X | lld:pubmed |
| pubmed-article:11162874 | pubmed:author | pubmed-author:TchaoRR | lld:pubmed |
| pubmed-article:11162874 | pubmed:author | pubmed-author:TarloffJ BJB | lld:pubmed |
| pubmed-article:11162874 | pubmed:author | pubmed-author:HallmanM AMA | lld:pubmed |
| pubmed-article:11162874 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11162874 | pubmed:day | 7 | lld:pubmed |
| pubmed-article:11162874 | pubmed:volume | 156 | lld:pubmed |
| pubmed-article:11162874 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11162874 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11162874 | pubmed:pagination | 37-45 | lld:pubmed |
| pubmed-article:11162874 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:11162874 | pubmed:meshHeading | pubmed-meshheading:11162874... | lld:pubmed |
| pubmed-article:11162874 | pubmed:meshHeading | pubmed-meshheading:11162874... | lld:pubmed |
| pubmed-article:11162874 | pubmed:meshHeading | pubmed-meshheading:11162874... | lld:pubmed |
| pubmed-article:11162874 | pubmed:meshHeading | pubmed-meshheading:11162874... | lld:pubmed |
| pubmed-article:11162874 | pubmed:meshHeading | pubmed-meshheading:11162874... | lld:pubmed |
| pubmed-article:11162874 | pubmed:meshHeading | pubmed-meshheading:11162874... | lld:pubmed |
| pubmed-article:11162874 | pubmed:meshHeading | pubmed-meshheading:11162874... | lld:pubmed |
| pubmed-article:11162874 | pubmed:meshHeading | pubmed-meshheading:11162874... | lld:pubmed |
| pubmed-article:11162874 | pubmed:meshHeading | pubmed-meshheading:11162874... | lld:pubmed |
| pubmed-article:11162874 | pubmed:meshHeading | pubmed-meshheading:11162874... | lld:pubmed |
| pubmed-article:11162874 | pubmed:meshHeading | pubmed-meshheading:11162874... | lld:pubmed |
| pubmed-article:11162874 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:11162874 | pubmed:articleTitle | Effect of antioxidants on para-aminophenol-induced toxicity in LLC-PK1 cells. | lld:pubmed |
| pubmed-article:11162874 | pubmed:affiliation | Department of Pharmaceutical Sciences, University of the Science in Philadelphia, 600 South 43rd Street, Philadelphia, PA 19104-4495, USA. | lld:pubmed |
| pubmed-article:11162874 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11162874 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11162874 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11162874 | lld:pubmed |
