| pubmed-article:11159435 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11159435 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:11159435 | lifeskim:mentions | umls-concept:C0699733 | lld:lifeskim |
| pubmed-article:11159435 | lifeskim:mentions | umls-concept:C0441722 | lld:lifeskim |
| pubmed-article:11159435 | lifeskim:mentions | umls-concept:C1516048 | lld:lifeskim |
| pubmed-article:11159435 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:11159435 | pubmed:dateCreated | 2001-2-22 | lld:pubmed |
| pubmed-article:11159435 | pubmed:abstractText | A microcantilever technique was used to apply force to receptor-ligand molecules involved in leukocyte rolling on blood vessel walls. E-selectin was adsorbed onto 3-microm-diameter, 4-mm-long glass fibers, and the selectin ligand, sialyl Lewis(x), was coupled to latex microspheres. After binding, the microsphere and bound fiber were retracted using a computerized loading protocol that combines hydrodynamic and Hookean forces on the fiber to produce a range of force loading rates (force/time), r(f). From the distribution of forces at failure, the average force was determined and plotted as a function of ln r(f). The slope and intercept of the plot yield the unstressed reverse reaction rate, k(r)(o), and a parameter that describes the force dependence of reverse reaction rates, r(o). The ligand was titrated so adhesion occurred in approximately 30% of tests, implying that >80% of adhesive events involve single bonds. Monte Carlo simulations show that this level of multiple bonding has little effect on parameter estimation. The estimates are r(o) = 0.048 and 0.016 nm and k(r)(o) = 0.72 and 2.2 s(-1) for loading rates in the ranges 200-1000 and 1000-5000 pN s(-1), respectively. Levenberg-Marquardt fitting across all values of r(f) gives r(o) = 0.034 nm and k(r)(o) = 0.82 s(-1). The values of these parameters are in the range required for rolling, as suggested by adhesive dynamics simulations. | lld:pubmed |
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| pubmed-article:11159435 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:11159435 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:11159435 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11159435 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:11159435 | pubmed:issn | 0006-3495 | lld:pubmed |
| pubmed-article:11159435 | pubmed:author | pubmed-author:HammerD ADA | lld:pubmed |
| pubmed-article:11159435 | pubmed:author | pubmed-author:WaughR ERE | lld:pubmed |
| pubmed-article:11159435 | pubmed:author | pubmed-author:TeesD FDF | lld:pubmed |
| pubmed-article:11159435 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11159435 | pubmed:volume | 80 | lld:pubmed |
| pubmed-article:11159435 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11159435 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11159435 | pubmed:pagination | 668-82 | lld:pubmed |
| pubmed-article:11159435 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:11159435 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11159435 | pubmed:articleTitle | A microcantilever device to assess the effect of force on the lifetime of selectin-carbohydrate bonds. | lld:pubmed |
| pubmed-article:11159435 | pubmed:affiliation | Department of Chemical Engineering and Institute of Medicine and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA. | lld:pubmed |
| pubmed-article:11159435 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11159435 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:11159435 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:11159435 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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