| pubmed-article:11141170 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11141170 | lifeskim:mentions | umls-concept:C0162740 | lld:lifeskim |
| pubmed-article:11141170 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:11141170 | lifeskim:mentions | umls-concept:C0001830 | lld:lifeskim |
| pubmed-article:11141170 | lifeskim:mentions | umls-concept:C0017431 | lld:lifeskim |
| pubmed-article:11141170 | lifeskim:mentions | umls-concept:C0596988 | lld:lifeskim |
| pubmed-article:11141170 | lifeskim:mentions | umls-concept:C0032082 | lld:lifeskim |
| pubmed-article:11141170 | lifeskim:mentions | umls-concept:C0086035 | lld:lifeskim |
| pubmed-article:11141170 | lifeskim:mentions | umls-concept:C1517499 | lld:lifeskim |
| pubmed-article:11141170 | pubmed:issue | 353 | lld:pubmed |
| pubmed-article:11141170 | pubmed:dateCreated | 2001-1-26 | lld:pubmed |
| pubmed-article:11141170 | pubmed:abstractText | Many plant species and/or genotypes are highly recalcitrant to Agrobacterium-mediated genetic transformation, and yet little is known about this phenomenon. Using several Arabidopsis genotypes/ecotypes, the results of this study indicated that phytohormone pretreatment could overcome this recalcitrance by increasing the transformation rate in the known recalcitrant genotypes. Transient expression of a T-DNA encoded ss-glucuronidase (GUS) gene and stable kanamycin resistance were obtained for the ten Arabidopsis genotypes tested as well as for the mutant uvh1 (up to 69% of petioles with blue spots and up to 42% resistant calli). Cultivation of Arabidopsis tissues on phytohormones for 2-8 d before co-cultivation with Agrobacterium tumefaciens significantly increased transient GUS gene expression by 2-11-fold and stable T-DNA integration with petiole explants. Different Arabidopsis ecotypes revealed differences in their susceptibility to Agrobacterium-mediated transformation and in their type of reaction to pre-cultivation (three types of reactions were defined by gathering ecotypes into three groups). The Arabidopsis uvh1 mutant described as defective in a DNA repair system showed slightly lower competence to transformation than did its progenitor Colombia. This reduced transformation competence, however, could be overcome by 4-d pre-culture with phytohormones. The importance of pre-cultivation with phytohormones for genetic transformation is discussed. | lld:pubmed |
| pubmed-article:11141170 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11141170 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11141170 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11141170 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11141170 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11141170 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11141170 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11141170 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:11141170 | pubmed:issn | 0022-0957 | lld:pubmed |
| pubmed-article:11141170 | pubmed:author | pubmed-author:SangwanR SRS | lld:pubmed |
| pubmed-article:11141170 | pubmed:author | pubmed-author:Sangwan-Norre... | lld:pubmed |
| pubmed-article:11141170 | pubmed:author | pubmed-author:ChateauSS | lld:pubmed |
| pubmed-article:11141170 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11141170 | pubmed:volume | 51 | lld:pubmed |
| pubmed-article:11141170 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11141170 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11141170 | pubmed:pagination | 1961-8 | lld:pubmed |
| pubmed-article:11141170 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:11141170 | pubmed:meshHeading | pubmed-meshheading:11141170... | lld:pubmed |
| pubmed-article:11141170 | pubmed:meshHeading | pubmed-meshheading:11141170... | lld:pubmed |
| pubmed-article:11141170 | pubmed:meshHeading | pubmed-meshheading:11141170... | lld:pubmed |
| pubmed-article:11141170 | pubmed:meshHeading | pubmed-meshheading:11141170... | lld:pubmed |
| pubmed-article:11141170 | pubmed:meshHeading | pubmed-meshheading:11141170... | lld:pubmed |
| pubmed-article:11141170 | pubmed:meshHeading | pubmed-meshheading:11141170... | lld:pubmed |
| pubmed-article:11141170 | pubmed:meshHeading | pubmed-meshheading:11141170... | lld:pubmed |
| pubmed-article:11141170 | pubmed:meshHeading | pubmed-meshheading:11141170... | lld:pubmed |
| pubmed-article:11141170 | pubmed:meshHeading | pubmed-meshheading:11141170... | lld:pubmed |
| pubmed-article:11141170 | pubmed:meshHeading | pubmed-meshheading:11141170... | lld:pubmed |
| pubmed-article:11141170 | pubmed:meshHeading | pubmed-meshheading:11141170... | lld:pubmed |
| pubmed-article:11141170 | pubmed:meshHeading | pubmed-meshheading:11141170... | lld:pubmed |
| pubmed-article:11141170 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:11141170 | pubmed:articleTitle | Competence of Arabidopsis thaliana genotypes and mutants for Agrobacterium tumefaciens-mediated gene transfer: role of phytohormones. | lld:pubmed |
| pubmed-article:11141170 | pubmed:affiliation | Laboratoire Androgenèse et Biotechnologie, Université de Picardie Jules Verne, 33 rue Saint-Leu, 80039 Amiens cedex 01, France. | lld:pubmed |
| pubmed-article:11141170 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11141170 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
