Linked Life Data

11097635

Source:http://linkedlifedata.com/resource/pubmed/id/11097635

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:11097635rdf:typepubmed:Citationlld:pubmed
pubmed-article:11097635lifeskim:mentionsumls-concept:C0034493lld:lifeskim
pubmed-article:11097635lifeskim:mentionsumls-concept:C0687028lld:lifeskim
pubmed-article:11097635lifeskim:mentionsumls-concept:C0007776lld:lifeskim
pubmed-article:11097635lifeskim:mentionsumls-concept:C0022655lld:lifeskim
pubmed-article:11097635lifeskim:mentionsumls-concept:C0001613lld:lifeskim
pubmed-article:11097635lifeskim:mentionsumls-concept:C0035930lld:lifeskim
pubmed-article:11097635lifeskim:mentionsumls-concept:C1516698lld:lifeskim
pubmed-article:11097635lifeskim:mentionsumls-concept:C0243144lld:lifeskim
pubmed-article:11097635pubmed:issue6lld:pubmed
pubmed-article:11097635pubmed:dateCreated2001-1-3lld:pubmed
pubmed-article:11097635pubmed:abstractTextWithin the renal cortical collecting duct (CCD), transepithelial Na(+) absorption and K(+) secretion are linked to basolateral Na(+)-K(+)-ATPase activity. Our purpose was to examine the developmental changes in basolateral Na(+)-K(+)-ATPase-mediated (86)rubidium (Rb) uptake, its inhibitor sensitivity and relationship to pump hydrolytic activity and Na(+) transport. Multiple CCDs ( approximately 6 mm) from maturing rabbits were affixed to coverslips, preincubated at 37 degrees C for 10 min (+/-1-2.5 mM ouabain or 10 or 100 micro M Schering-28080, an inhibitor of H(+)-K(+)-ATPase), and then transferred to prewarmed incubation solution containing tracer amounts of (86)Rb (+/-inhibitors). After 1 min at 37 degrees C, tubular samples were rinsed and permeabilized and isotope counts were measured to calculate basolateral Rb uptake. Ouabain-inhibitable Rb uptake, an index of basolateral Na(+)-K(+) pump activity, increased approximately 3-fold during the 1st 8 wk of postnatal life (P < 0.03). The approximately 2-fold increase in absolute rate of Rb uptake between 1 and 6 wk (2.64 +/- 0.45 to 5.02 +/- 0.32 pmol. min(-1). mm(-1)) did not reach statistical significance. The rate of basolateral Rb uptake increased further after the 6th wk of life to 7.29 +/- 0.53 pmol. min(-1). mm(-1) in adult animals (P < 0.03 vs. 6 wk). Schering-28080 failed to inhibit Rb uptake, implying that functional H(+)-K(+)-ATPase is absent at the basolateral membrane. Na(+)-K(+)-ATPase hydrolytic activity, determined by using a microassay that measured inorganic phosphate release from [gamma-(32)P]ATP under maximum velocity (V(max)) conditions, also increased in the differentiating CCD (from 316.2 +/- 44.4 pmol. h(-1). mm(-1) at 2 wk to 555.9 +/- 105.1 at 4 wk to 789.7 +/- 145.0 at 6 wk; r = 1.0 by linear regression analysis; P < 0.005). The parallel approximately 2.5-fold increases in Na(+)-K(+)-ATPase activity and ouabain-sensitive Rb uptake between 2- and 6-wk postnatal age suggest that the developmental increase in basolateral transport capacity is due predominantly to an increase in enzyme abundance. The signals mediating the developmental increase in Na(+)-K(+)-ATPase activity in the CCD remain to be defined.lld:pubmed
pubmed-article:11097635pubmed:granthttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11097635pubmed:languageenglld:pubmed
pubmed-article:11097635pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11097635pubmed:citationSubsetIMlld:pubmed
pubmed-article:11097635pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11097635pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11097635pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11097635pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11097635pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11097635pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11097635pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11097635pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11097635pubmed:statusMEDLINElld:pubmed
pubmed-article:11097635pubmed:monthDeclld:pubmed
pubmed-article:11097635pubmed:issn1931-857Xlld:pubmed
pubmed-article:11097635pubmed:authorpubmed-author:LaneJ CJClld:pubmed
pubmed-article:11097635pubmed:authorpubmed-author:MaiHHlld:pubmed
pubmed-article:11097635pubmed:authorpubmed-author:SatlinL MLMlld:pubmed
pubmed-article:11097635pubmed:authorpubmed-author:ZavilowitzBBlld:pubmed
pubmed-article:11097635pubmed:authorpubmed-author:Constantinesc...lld:pubmed
pubmed-article:11097635pubmed:issnTypePrintlld:pubmed
pubmed-article:11097635pubmed:volume279lld:pubmed
pubmed-article:11097635pubmed:ownerNLMlld:pubmed
pubmed-article:11097635pubmed:authorsCompleteYlld:pubmed
pubmed-article:11097635pubmed:paginationF1161-8lld:pubmed
pubmed-article:11097635pubmed:dateRevised2011-4-28lld:pubmed
pubmed-article:11097635pubmed:meshHeadingpubmed-meshheading:11097635...lld:pubmed
pubmed-article:11097635pubmed:meshHeadingpubmed-meshheading:11097635...lld:pubmed
pubmed-article:11097635pubmed:meshHeadingpubmed-meshheading:11097635...lld:pubmed
pubmed-article:11097635pubmed:meshHeadingpubmed-meshheading:11097635...lld:pubmed
pubmed-article:11097635pubmed:meshHeadingpubmed-meshheading:11097635...lld:pubmed
pubmed-article:11097635pubmed:meshHeadingpubmed-meshheading:11097635...lld:pubmed
pubmed-article:11097635pubmed:meshHeadingpubmed-meshheading:11097635...lld:pubmed
pubmed-article:11097635pubmed:meshHeadingpubmed-meshheading:11097635...lld:pubmed
pubmed-article:11097635pubmed:meshHeadingpubmed-meshheading:11097635...lld:pubmed
pubmed-article:11097635pubmed:meshHeadingpubmed-meshheading:11097635...lld:pubmed
pubmed-article:11097635pubmed:meshHeadingpubmed-meshheading:11097635...lld:pubmed
pubmed-article:11097635pubmed:meshHeadingpubmed-meshheading:11097635...lld:pubmed
pubmed-article:11097635pubmed:meshHeadingpubmed-meshheading:11097635...lld:pubmed
pubmed-article:11097635pubmed:meshHeadingpubmed-meshheading:11097635...lld:pubmed
pubmed-article:11097635pubmed:year2000lld:pubmed
pubmed-article:11097635pubmed:articleTitleNa(+)-K(+)-ATPase-mediated basolateral rubidium uptake in the maturing rabbit cortical collecting duct.lld:pubmed
pubmed-article:11097635pubmed:affiliationDepartment of Pediatrics, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, New Brunswick, New Jersey, USA.lld:pubmed
pubmed-article:11097635pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:11097635pubmed:publicationTypeIn Vitrolld:pubmed
pubmed-article:11097635pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
pubmed-article:11097635pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed