| pubmed-article:11052809 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11052809 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:11052809 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:11052809 | lifeskim:mentions | umls-concept:C0016055 | lld:lifeskim |
| pubmed-article:11052809 | lifeskim:mentions | umls-concept:C0021753 | lld:lifeskim |
| pubmed-article:11052809 | lifeskim:mentions | umls-concept:C0085828 | lld:lifeskim |
| pubmed-article:11052809 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:11052809 | lifeskim:mentions | umls-concept:C0033634 | lld:lifeskim |
| pubmed-article:11052809 | lifeskim:mentions | umls-concept:C1158770 | lld:lifeskim |
| pubmed-article:11052809 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:11052809 | pubmed:dateCreated | 2000-12-18 | lld:pubmed |
| pubmed-article:11052809 | pubmed:abstractText | Interleukin 1beta (IL-1beta) is a multifunctional polypeptide considered a key cytokine during inflammation. Fibronectin (FN), a matrix glycoprotein highly expressed in injured tissues, can induce expression of IL-1beta in human blood monocytic cells. Herein, we explore the intracellular signals and transcriptional mechanisms responsible for IL-1beta induction by FN using human promonocytic U937 cells transfected with the human IL-1beta promoter connected to a reporter gene. Exposure of transfected U937s to FN resulted in increased expression of the full-length IL-1beta promoter. This effect, mediated via the alpha5beta1 integrin, was associated with activation of mitogen-activated protein kinases (MAPKs) and was abolished by pre-treatment of cells with Calphostin C, a specific inhibitor of protein kinase C (PKC) activation. Deletion analysis and co-transfection studies using consensus activator protein 1 (AP-1) oligonucleotides suggested that an AP-1 site present in the 5' end of the IL-1beta promoter was involved in the FN-induced response. Finally, electrophoretic mobility shift assays showed that FN induced binding of AP-1, but not NF-kappaB. Together, these experiments demonstrate that FN binding to the alpha5beta1 integrin activates MAPK-dependent signal pathways, and results in the transcription of the IL-1beta promoter in U937 cells by activating PKC and inducing AP-1. | lld:pubmed |
| pubmed-article:11052809 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11052809 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11052809 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11052809 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11052809 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11052809 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11052809 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11052809 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11052809 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11052809 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11052809 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11052809 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11052809 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11052809 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11052809 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11052809 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11052809 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11052809 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11052809 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11052809 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11052809 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11052809 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11052809 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11052809 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:11052809 | pubmed:issn | 1043-4666 | lld:pubmed |
| pubmed-article:11052809 | pubmed:author | pubmed-author:RomanJJ | lld:pubmed |
| pubmed-article:11052809 | pubmed:author | pubmed-author:FentonM JMJ | lld:pubmed |
| pubmed-article:11052809 | pubmed:author | pubmed-author:RitzenthalerJ... | lld:pubmed |
| pubmed-article:11052809 | pubmed:author | pubmed-author:RoserSS | lld:pubmed |
| pubmed-article:11052809 | pubmed:author | pubmed-author:SchuylerWW | lld:pubmed |
| pubmed-article:11052809 | pubmed:copyrightInfo | Copyright 2000 Academic Press. | lld:pubmed |
| pubmed-article:11052809 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11052809 | pubmed:volume | 12 | lld:pubmed |
| pubmed-article:11052809 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11052809 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11052809 | pubmed:pagination | 1581-96 | lld:pubmed |
| pubmed-article:11052809 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:11052809 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:11052809 | pubmed:articleTitle | Transcriptional regulation of the human interleukin 1beta gene by fibronectin: role of protein kinase C and activator protein 1 (AP-1). | lld:pubmed |
| pubmed-article:11052809 | pubmed:affiliation | Pulmonary and Critical Care Division, Department of Medicine, Atlanta Veterans Affairs Medical Center and Emory University School of Medicine, Atlanta, Georgia, USA. roman-rodriguez.jesse@atlanta.va.gov | lld:pubmed |
| pubmed-article:11052809 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11052809 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:11052809 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11052809 | lld:pubmed |
