| pubmed-article:11001365 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11001365 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:11001365 | lifeskim:mentions | umls-concept:C0011306 | lld:lifeskim |
| pubmed-article:11001365 | lifeskim:mentions | umls-concept:C0020964 | lld:lifeskim |
| pubmed-article:11001365 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:11001365 | pubmed:issue | 17 | lld:pubmed |
| pubmed-article:11001365 | pubmed:dateCreated | 2000-10-6 | lld:pubmed |
| pubmed-article:11001365 | pubmed:abstractText | Dendritic cells are professional antigen-presenting cells able to prime naive T lymphocytes and regulate steadily the delicate balance between tolerance and activation during the immune response. In past years several reports have shown that genetically engineered dendritic cells (DCs) can be a powerful tool for inducing an antigen-specific immune response. The use of such modified antigen-presenting cells is a real working hypothesis in preclinical studies and in clinical vaccination approaches for cancer treatment. The definition of optimal transfection conditions for preserving DC survival and functionality is necessary to design a correct immunotherapeutic protocol. Different lipid-based transfection compounds were studied for their effects on DC survival, phenotype and functional properties. All the transfection procedures were able to select DCs with a higher expression of activation and costimulatory molecules (ie MHCII-DR, CD83, CD86, CD25) than the untreated DCs. However, only two compounds (LipofectAMINE PLUS and FuGENE 6), preserved or even increased the immunopotency of DCs as antigen-presenting cells. These protocols were applied to modify DCs in order to express an epithelial tumor-associated antigen, MUC1, and such cells were able to induce in vitro a specific immune response in healthy donors. | lld:pubmed |
| pubmed-article:11001365 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11001365 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11001365 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11001365 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11001365 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11001365 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11001365 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11001365 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11001365 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11001365 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11001365 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11001365 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11001365 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11001365 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:11001365 | pubmed:issn | 0969-7128 | lld:pubmed |
| pubmed-article:11001365 | pubmed:author | pubmed-author:NutiMM | lld:pubmed |
| pubmed-article:11001365 | pubmed:author | pubmed-author:RahimiHH | lld:pubmed |
| pubmed-article:11001365 | pubmed:author | pubmed-author:FratiLL | lld:pubmed |
| pubmed-article:11001365 | pubmed:author | pubmed-author:FattorossiAA | lld:pubmed |
| pubmed-article:11001365 | pubmed:author | pubmed-author:RughettiAA | lld:pubmed |
| pubmed-article:11001365 | pubmed:author | pubmed-author:ScambiaGG | lld:pubmed |
| pubmed-article:11001365 | pubmed:author | pubmed-author:PierelliLL | lld:pubmed |
| pubmed-article:11001365 | pubmed:author | pubmed-author:BiffoniMM | lld:pubmed |
| pubmed-article:11001365 | pubmed:author | pubmed-author:LavitranoMM | lld:pubmed |
| pubmed-article:11001365 | pubmed:author | pubmed-author:PellicciottiA... | lld:pubmed |
| pubmed-article:11001365 | pubmed:author | pubmed-author:SabbatucciMM | lld:pubmed |
| pubmed-article:11001365 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11001365 | pubmed:volume | 7 | lld:pubmed |
| pubmed-article:11001365 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11001365 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11001365 | pubmed:pagination | 1458-66 | lld:pubmed |
| pubmed-article:11001365 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:11001365 | pubmed:meshHeading | pubmed-meshheading:11001365... | lld:pubmed |
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| pubmed-article:11001365 | pubmed:meshHeading | pubmed-meshheading:11001365... | lld:pubmed |
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| pubmed-article:11001365 | pubmed:meshHeading | pubmed-meshheading:11001365... | lld:pubmed |
| pubmed-article:11001365 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:11001365 | pubmed:articleTitle | Transfected human dendritic cells to induce antitumor immunity. | lld:pubmed |
| pubmed-article:11001365 | pubmed:affiliation | Department of Experimental Medicine and Pathology, Università di Roma La Sapienza, Italy. | lld:pubmed |
| pubmed-article:11001365 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11001365 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11001365 | lld:pubmed |
