| pubmed-article:10945858 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10945858 | lifeskim:mentions | umls-concept:C0034493 | lld:lifeskim |
| pubmed-article:10945858 | lifeskim:mentions | umls-concept:C0027061 | lld:lifeskim |
| pubmed-article:10945858 | lifeskim:mentions | umls-concept:C0225828 | lld:lifeskim |
| pubmed-article:10945858 | lifeskim:mentions | umls-concept:C1522565 | lld:lifeskim |
| pubmed-article:10945858 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
| pubmed-article:10945858 | lifeskim:mentions | umls-concept:C0079284 | lld:lifeskim |
| pubmed-article:10945858 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:10945858 | lifeskim:mentions | umls-concept:C0086597 | lld:lifeskim |
| pubmed-article:10945858 | lifeskim:mentions | umls-concept:C0449560 | lld:lifeskim |
| pubmed-article:10945858 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:10945858 | pubmed:dateCreated | 2000-9-12 | lld:pubmed |
| pubmed-article:10945858 | pubmed:abstractText | Endothelin (ET)-1 is a potent positive inotropic agent, the effects of which are mediated by increases in cytosolic Ca(2+) in the myocardium. The object of this study was to examine 1) the influence of ET(A) and ET(B) receptor subtypes, and 2) the role of the phospholipase C (PLC) pathway in mediating ET-1-induced contraction. Left ventricular cardiomyocytes were isolated from the hearts of New Zealand White rabbits (2-2.5 kg) by the use of Langendorff perfusion with collagenase. Cardiomyocyte function was examined during unloaded, electrically stimulated (0.5 Hz) contractions with a video-edge detection system. ET-1 increased cell shortening with greater potency than ET-3: mean EC(50) values were 1.1 x 10(-11) and 2.6 x 10(-10) M, respectively. With the same order of potency, ET-1 and ET-3 increased (P <.05) velocity of cell shortening. The ET(A) receptor-selective antagonist ABT-627 shifted the ET-1-induced cell shortening response curve to the right with a pA(2) value of 10.3. The ET(B) receptor-selective antagonist A-192621 (10(-8)-10(-7) M) did not alter the concentration-response of ET-1. Moreover, the ET(B) receptor-selective agonist sarafotoxin 6c did not have any effect on cell shortening over the concentration range of 10(-11) to 10(-7) M. ET-1 in the presence of the PLC inhibitor U-73122 did not alter the contractile amplitude. However, ET-1 in the presence of the protein kinase C inhibitor bisindolylmalemide increased cell shortening. These findings indicate that 1) the ET(A) receptor subtype, and not the ET(B) receptor subtype, mediates the positive inotropic effect of ET-1, and 2) the response of ET-1 is mediated by a PLC pathway, but not through protein kinase C, in ventricular cardiomyocytes isolated from rabbit myocardium. | lld:pubmed |
| pubmed-article:10945858 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10945858 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10945858 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10945858 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10945858 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10945858 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10945858 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10945858 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10945858 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10945858 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10945858 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10945858 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10945858 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10945858 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10945858 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:10945858 | pubmed:issn | 0022-3565 | lld:pubmed |
| pubmed-article:10945858 | pubmed:author | pubmed-author:McDermottB... | lld:pubmed |
| pubmed-article:10945858 | pubmed:author | pubmed-author:SilkeBB | lld:pubmed |
| pubmed-article:10945858 | pubmed:author | pubmed-author:SpiersJ PJP | lld:pubmed |
| pubmed-article:10945858 | pubmed:author | pubmed-author:KelsoE JEJ | lld:pubmed |
| pubmed-article:10945858 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10945858 | pubmed:volume | 294 | lld:pubmed |
| pubmed-article:10945858 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10945858 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10945858 | pubmed:pagination | 1047-52 | lld:pubmed |
| pubmed-article:10945858 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:10945858 | pubmed:meshHeading | pubmed-meshheading:10945858... | lld:pubmed |
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| pubmed-article:10945858 | pubmed:meshHeading | pubmed-meshheading:10945858... | lld:pubmed |
| pubmed-article:10945858 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10945858 | pubmed:articleTitle | Endothelin(A) receptor subtype mediates endothelin-induced contractility in left ventricular cardiomyocytes isolated from rabbit myocardium. | lld:pubmed |
| pubmed-article:10945858 | pubmed:affiliation | Cardiovascular Pharmacology Group, Centre for Cardiovascular Research, School of Medicine, The Queen's University of Belfast, Northern Ireland, United Kingdom. e.kelso@qub.ac.uk | lld:pubmed |
| pubmed-article:10945858 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10945858 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:10945858 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10945858 | lld:pubmed |
