| pubmed-article:10938903 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10938903 | lifeskim:mentions | umls-concept:C0006675 | lld:lifeskim |
| pubmed-article:10938903 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:10938903 | lifeskim:mentions | umls-concept:C0005821 | lld:lifeskim |
| pubmed-article:10938903 | lifeskim:mentions | umls-concept:C0178719 | lld:lifeskim |
| pubmed-article:10938903 | lifeskim:mentions | umls-concept:C0040018 | lld:lifeskim |
| pubmed-article:10938903 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:10938903 | lifeskim:mentions | umls-concept:C0591833 | lld:lifeskim |
| pubmed-article:10938903 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:10938903 | pubmed:dateCreated | 2000-9-19 | lld:pubmed |
| pubmed-article:10938903 | pubmed:abstractText | The aim of this study was to compare the effect of thrombin (Thr) on cytosolic calcium [Ca2]+i and intracellular pH [pH]i in human and murine platelets. Rich-platelet suspensions from both species were loaded with Fura-2 (2 microM) or BCECF (0.75 microM) by incubation with their respective acetoxymethyl esters to measure cytosolic calcium [Ca2+]i or intracellular pH [pH]i, respectively. Suspensions were challenged with increasing concentrations of Thr, from 0.1 to 10 IU/ml. Basal [Ca2+]i in human platelets was 98 +/- 6 and 99.1 +/- 9 nM in rat platelets (n = 20). Thr increased [Ca2+]i, EC50 was 1.1 +/- 0.04 in human and 0.97 +/- 0.06 IU/ml in rat platelets (n = 7). Extracellular Mg2+ (4 or 8 mM) abolished Thr response on [Ca2+]i. [pH]i in human was 7.09 +/- 0.08 and 7.11 +/- 0.04 in rat platelets. Thr induced alkalinization of platelets in both species. Our results indicate that the potency of Thr to change [Ca2+]i and [pH]i was similar in both species, allowing for comparisons between human and murine platelets and to extrapolate results from an animal model to human pathology. | lld:pubmed |
| pubmed-article:10938903 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10938903 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10938903 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10938903 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10938903 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10938903 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10938903 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10938903 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10938903 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10938903 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10938903 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10938903 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:10938903 | pubmed:issn | 0953-7104 | lld:pubmed |
| pubmed-article:10938903 | pubmed:author | pubmed-author:PaniaguaRR | lld:pubmed |
| pubmed-article:10938903 | pubmed:author | pubmed-author:SalazarDD | lld:pubmed |
| pubmed-article:10938903 | pubmed:author | pubmed-author:SierraGG | lld:pubmed |
| pubmed-article:10938903 | pubmed:author | pubmed-author:ReyesJ LJL | lld:pubmed |
| pubmed-article:10938903 | pubmed:author | pubmed-author:MelendezEE | lld:pubmed |
| pubmed-article:10938903 | pubmed:author | pubmed-author:ValenciaLL | lld:pubmed |
| pubmed-article:10938903 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10938903 | pubmed:volume | 11 | lld:pubmed |
| pubmed-article:10938903 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10938903 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10938903 | pubmed:pagination | 233-40 | lld:pubmed |
| pubmed-article:10938903 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:10938903 | pubmed:meshHeading | pubmed-meshheading:10938903... | lld:pubmed |
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| pubmed-article:10938903 | pubmed:meshHeading | pubmed-meshheading:10938903... | lld:pubmed |
| pubmed-article:10938903 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10938903 | pubmed:articleTitle | Effects of thrombin on intracellular calcium and pH in human and murine platelets. | lld:pubmed |
| pubmed-article:10938903 | pubmed:affiliation | Department of Physiology and Biophysics, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, D.F., Mexico. | lld:pubmed |
| pubmed-article:10938903 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10938903 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:10938903 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
