| pubmed-article:10919277 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10919277 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:10919277 | lifeskim:mentions | umls-concept:C0171961 | lld:lifeskim |
| pubmed-article:10919277 | lifeskim:mentions | umls-concept:C1335858 | lld:lifeskim |
| pubmed-article:10919277 | lifeskim:mentions | umls-concept:C2698421 | lld:lifeskim |
| pubmed-article:10919277 | lifeskim:mentions | umls-concept:C1417838 | lld:lifeskim |
| pubmed-article:10919277 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:10919277 | lifeskim:mentions | umls-concept:C0290758 | lld:lifeskim |
| pubmed-article:10919277 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:10919277 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:10919277 | pubmed:dateCreated | 2000-8-16 | lld:pubmed |
| pubmed-article:10919277 | pubmed:abstractText | Steroidogenic acute regulatory (StAR) protein plays a critical role in the movement of cholesterol from the outer to the inner mitochondrial membrane. Steroidogenic factor 1 (SF-1) controls basal and cAMP-stimulated transcription of the StAR gene. The 1.3-kb StAR promoter has three SF-1 binding sites, and two consensus transcription factor Spl binding sequences near the two most distal SF-1 binding sites. Spl mediates cAMP-dependent transcription of steroidogenic P450 enzyme genes, raising the possibility of Sp1 involvement in cAMP regulation of the StAR gene. However, the mechanism of Spl-mediated, cAMP-stimulated responsiveness is not known. In this study, we elucidated the roles of Sp1 and SF-1 in the regulation of the human StAR gene promoter. We found that there was negligible promoter activity in a pGL2 StAR construct (-235 to +39) in which Spl and SF-1 binding sites were mutated in Y-1 adrenal tumor cells. An Sp1 binding site mutation (pGL2Sp1M) did not support promoter activity, suggesting that Spl cooperates with SF-1 in regulating StAR promoter function. In gel shift assays, the SF-1 binding site formed a complex with an SF-1-GST fusion protein and Spl. Coimmunoprecipitation cross-linking experiments indicated that SF-1 physically interacts with Sp1 in vitro. Finally, a mammalian two-hybrid system was employed to demonstrate that Spl and SF-1 associate in vivo. In conclusion, our data indicate that Spl and SF-1 physically interact and cooperate in the regulation of human StAR promoter activity. | lld:pubmed |
| pubmed-article:10919277 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10919277 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10919277 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:10919277 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10919277 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10919277 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10919277 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10919277 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:10919277 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10919277 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10919277 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10919277 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10919277 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10919277 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10919277 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:10919277 | pubmed:issn | 0013-7227 | lld:pubmed |
| pubmed-article:10919277 | pubmed:author | pubmed-author:SaitoMM | lld:pubmed |
| pubmed-article:10919277 | pubmed:author | pubmed-author:FujimotoSS | lld:pubmed |
| pubmed-article:10919277 | pubmed:author | pubmed-author:SugawaraTT | lld:pubmed |
| pubmed-article:10919277 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10919277 | pubmed:volume | 141 | lld:pubmed |
| pubmed-article:10919277 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10919277 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10919277 | pubmed:pagination | 2895-903 | lld:pubmed |
| pubmed-article:10919277 | pubmed:dateRevised | 2009-8-12 | lld:pubmed |
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| pubmed-article:10919277 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10919277 | pubmed:articleTitle | Sp1 and SF-1 interact and cooperate in the regulation of human steroidogenic acute regulatory protein gene expression. | lld:pubmed |
| pubmed-article:10919277 | pubmed:affiliation | Department of Biochemistry, Hokkaido University School of Medicine, Sapporo, Japan. terusuga@med.hokudai.ac.jp | lld:pubmed |
| pubmed-article:10919277 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10919277 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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