| pubmed-article:10898588 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10898588 | lifeskim:mentions | umls-concept:C0596040 | lld:lifeskim |
| pubmed-article:10898588 | lifeskim:mentions | umls-concept:C0023775 | lld:lifeskim |
| pubmed-article:10898588 | lifeskim:mentions | umls-concept:C1441547 | lld:lifeskim |
| pubmed-article:10898588 | lifeskim:mentions | umls-concept:C1514468 | lld:lifeskim |
| pubmed-article:10898588 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:10898588 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:10898588 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:10898588 | lifeskim:mentions | umls-concept:C0911983 | lld:lifeskim |
| pubmed-article:10898588 | lifeskim:mentions | umls-concept:C0045183 | lld:lifeskim |
| pubmed-article:10898588 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:10898588 | pubmed:dateCreated | 2000-9-12 | lld:pubmed |
| pubmed-article:10898588 | pubmed:abstractText | Analysis of the reaction between 2'-deoxyadenosine and 4-oxo-2-nonenal by liquid chromatography/mass spectrometry revealed the presence of three major products (adducts A(1), A(2), and B). Adducts A(1) and A(2) were isomeric; they interconverted at room temperature, and they each readily dehydrated to form adduct B. The mass spectral characteristics of adduct B obtained by collision-induced dissociation coupled with multiple tandem mass spectrometry were consistent with those expected for a substituted etheno adduct. The structure of adduct B was shown by NMR spectroscopy to be consistent with the substituted etheno-2'-deoxyadenosine adduct 1' '-[3-(2'-deoxy-beta-D-erythropentafuranosyl)-3H-imidazo[2, 1-i]purin-7-yl]heptane-2' '-one. Unequivocal proof of structure came from the reaction of adducts A(1) and A(2) (precursors of adduct B) with sodium borohydride. Adducts A(1) and A(2) each formed the same reduction product, which contained eight additional hydrogen atoms. The mass spectral characteristics of this reduction product established that the exocyclic amino group (N(6)) of 2'-deoxyadenosine was attached to C-1 of the 4-oxo-2-nonenal. The reaction of 4-oxo-2-nonenal with calf thymus DNA was also shown to result in the formation of substituted ethano adducts A(1) and A(2) and substituted etheno adduct B. Adduct B was formed in amounts almost 2 orders of magnitude greater than those of adducts A(1) and A(2). This was in keeping with the observed stability of the adducts. The study presented here has provided additional evidence which shows that 4-oxo-2-nonenal reacts efficiently with DNA to form substituted etheno adducts. | lld:pubmed |
| pubmed-article:10898588 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10898588 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10898588 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10898588 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10898588 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10898588 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10898588 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10898588 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10898588 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10898588 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10898588 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10898588 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10898588 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:10898588 | pubmed:issn | 0893-228X | lld:pubmed |
| pubmed-article:10898588 | pubmed:author | pubmed-author:LeeS HSH | lld:pubmed |
| pubmed-article:10898588 | pubmed:author | pubmed-author:HajduEE | lld:pubmed |
| pubmed-article:10898588 | pubmed:author | pubmed-author:BlairI AIA | lld:pubmed |
| pubmed-article:10898588 | pubmed:author | pubmed-author:BibleR HRHJr | lld:pubmed |
| pubmed-article:10898588 | pubmed:author | pubmed-author:RindgenDD | lld:pubmed |
| pubmed-article:10898588 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10898588 | pubmed:volume | 13 | lld:pubmed |
| pubmed-article:10898588 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10898588 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10898588 | pubmed:pagination | 565-74 | lld:pubmed |
| pubmed-article:10898588 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:10898588 | pubmed:meshHeading | pubmed-meshheading:10898588... | lld:pubmed |
| pubmed-article:10898588 | pubmed:meshHeading | pubmed-meshheading:10898588... | lld:pubmed |
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| pubmed-article:10898588 | pubmed:meshHeading | pubmed-meshheading:10898588... | lld:pubmed |
| pubmed-article:10898588 | pubmed:meshHeading | pubmed-meshheading:10898588... | lld:pubmed |
| pubmed-article:10898588 | pubmed:meshHeading | pubmed-meshheading:10898588... | lld:pubmed |
| pubmed-article:10898588 | pubmed:meshHeading | pubmed-meshheading:10898588... | lld:pubmed |
| pubmed-article:10898588 | pubmed:meshHeading | pubmed-meshheading:10898588... | lld:pubmed |
| pubmed-article:10898588 | pubmed:meshHeading | pubmed-meshheading:10898588... | lld:pubmed |
| pubmed-article:10898588 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10898588 | pubmed:articleTitle | Characterization of 2'-deoxyadenosine adducts derived from 4-oxo-2-nonenal, a novel product of lipid peroxidation. | lld:pubmed |
| pubmed-article:10898588 | pubmed:affiliation | Center for Cancer Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104-6160, USA. | lld:pubmed |
| pubmed-article:10898588 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10898588 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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