Linked Life Data

10884060

Source:http://linkedlifedata.com/resource/pubmed/id/10884060

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:10884060rdf:typepubmed:Citationlld:pubmed
pubmed-article:10884060lifeskim:mentionsumls-concept:C0007634lld:lifeskim
pubmed-article:10884060lifeskim:mentionsumls-concept:C0034721lld:lifeskim
pubmed-article:10884060lifeskim:mentionsumls-concept:C0034693lld:lifeskim
pubmed-article:10884060lifeskim:mentionsumls-concept:C0521390lld:lifeskim
pubmed-article:10884060lifeskim:mentionsumls-concept:C0380603lld:lifeskim
pubmed-article:10884060lifeskim:mentionsumls-concept:C0038952lld:lifeskim
pubmed-article:10884060lifeskim:mentionsumls-concept:C0033414lld:lifeskim
pubmed-article:10884060lifeskim:mentionsumls-concept:C1517499lld:lifeskim
pubmed-article:10884060pubmed:issue9lld:pubmed
pubmed-article:10884060pubmed:dateCreated2000-10-10lld:pubmed
pubmed-article:10884060pubmed:abstractTextWe constructed two replication-deficient recombinant adenovirus vectors coding human basic fibroblast growth factor (bFGF), one with and one without the interleukin-2 (IL-2) secretory signal sequence and examined their neurotrophic effects on primary neuronal cells in vitro. The primary neuronal cells were successfully infected at a high efficiency with the adenovirus vectors. bFGF protein was detected in the culture medium of the neurons infected with both these vectors. The cells infected with the bFGF-expressing adenovirus containing the IL-2 signal sequence showed 2- to 10-fold higher levels of secretion levels than cells infected with the native bFGF-expressing adenovirus alone. Both bFGF-expressing vectors augmented the survival of primary neuronal cells in an in vitro culture, compared with a mock infection or control virus infection. Notably, the cells infected with the bFGF-expressing adenovirus containing the IL-2 signal sequence were markedly enhanced cell survival in the early phase of the culture, compared with the control cells and even those infected with the bFGF-expressing adenovirus without the IL-2 signal sequence. However, in the late phase of neuronal culture, neither viral vector could support the cell survival. In contrast the co-infection of the bFGF-expressing vector with a Bcl-xL-expressing vector was extremely effective on neuronal survival.lld:pubmed
pubmed-article:10884060pubmed:languageenglld:pubmed
pubmed-article:10884060pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:10884060pubmed:citationSubsetIMlld:pubmed
pubmed-article:10884060pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:10884060pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:10884060pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:10884060pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:10884060pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:10884060pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:10884060pubmed:statusMEDLINElld:pubmed
pubmed-article:10884060pubmed:monthJunlld:pubmed
pubmed-article:10884060pubmed:issn0959-4965lld:pubmed
pubmed-article:10884060pubmed:authorpubmed-author:MoriHHlld:pubmed
pubmed-article:10884060pubmed:authorpubmed-author:SaikiMMlld:pubmed
pubmed-article:10884060pubmed:authorpubmed-author:MatsuokaNNlld:pubmed
pubmed-article:10884060pubmed:authorpubmed-author:IshiiKKlld:pubmed
pubmed-article:10884060pubmed:authorpubmed-author:HashimotoNNlld:pubmed
pubmed-article:10884060pubmed:authorpubmed-author:HamadaHHlld:pubmed
pubmed-article:10884060pubmed:authorpubmed-author:YukawaHHlld:pubmed
pubmed-article:10884060pubmed:authorpubmed-author:AkimotoMMlld:pubmed
pubmed-article:10884060pubmed:authorpubmed-author:MiyatakeSSlld:pubmed
pubmed-article:10884060pubmed:authorpubmed-author:TakahashiJ...lld:pubmed
pubmed-article:10884060pubmed:issnTypePrintlld:pubmed
pubmed-article:10884060pubmed:day26lld:pubmed
pubmed-article:10884060pubmed:volume11lld:pubmed
pubmed-article:10884060pubmed:ownerNLMlld:pubmed
pubmed-article:10884060pubmed:authorsCompleteYlld:pubmed
pubmed-article:10884060pubmed:pagination2001-6lld:pubmed
pubmed-article:10884060pubmed:dateRevised2006-11-15lld:pubmed
pubmed-article:10884060pubmed:meshHeadingpubmed-meshheading:10884060...lld:pubmed
pubmed-article:10884060pubmed:meshHeadingpubmed-meshheading:10884060...lld:pubmed
pubmed-article:10884060pubmed:meshHeadingpubmed-meshheading:10884060...lld:pubmed
pubmed-article:10884060pubmed:meshHeadingpubmed-meshheading:10884060...lld:pubmed
pubmed-article:10884060pubmed:meshHeadingpubmed-meshheading:10884060...lld:pubmed
pubmed-article:10884060pubmed:meshHeadingpubmed-meshheading:10884060...lld:pubmed
pubmed-article:10884060pubmed:meshHeadingpubmed-meshheading:10884060...lld:pubmed
pubmed-article:10884060pubmed:meshHeadingpubmed-meshheading:10884060...lld:pubmed
pubmed-article:10884060pubmed:meshHeadingpubmed-meshheading:10884060...lld:pubmed
pubmed-article:10884060pubmed:meshHeadingpubmed-meshheading:10884060...lld:pubmed
pubmed-article:10884060pubmed:meshHeadingpubmed-meshheading:10884060...lld:pubmed
pubmed-article:10884060pubmed:meshHeadingpubmed-meshheading:10884060...lld:pubmed
pubmed-article:10884060pubmed:meshHeadingpubmed-meshheading:10884060...lld:pubmed
pubmed-article:10884060pubmed:meshHeadingpubmed-meshheading:10884060...lld:pubmed
pubmed-article:10884060pubmed:meshHeadingpubmed-meshheading:10884060...lld:pubmed
pubmed-article:10884060pubmed:year2000lld:pubmed
pubmed-article:10884060pubmed:articleTitleAdenovirus-mediated gene transfer of basic fibroblast growth factor promotes the survival of primary-cultured rat neuronal cells.lld:pubmed
pubmed-article:10884060pubmed:affiliationDepartment of Neurosurgery and Clinical Neuroscience, Faculty of Medicine, Kyoto University, Japan.lld:pubmed
pubmed-article:10884060pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:10884060pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:10884060lld:pubmed