| pubmed-article:10878037 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10878037 | lifeskim:mentions | umls-concept:C1265292 | lld:lifeskim |
| pubmed-article:10878037 | lifeskim:mentions | umls-concept:C0039421 | lld:lifeskim |
| pubmed-article:10878037 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:10878037 | lifeskim:mentions | umls-concept:C0020980 | lld:lifeskim |
| pubmed-article:10878037 | lifeskim:mentions | umls-concept:C0182400 | lld:lifeskim |
| pubmed-article:10878037 | lifeskim:mentions | umls-concept:C0439831 | lld:lifeskim |
| pubmed-article:10878037 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:10878037 | pubmed:dateCreated | 2000-9-1 | lld:pubmed |
| pubmed-article:10878037 | pubmed:abstractText | A Cycling Probe Technology (CPT) assay with a lateral-flow device (strip) was developed for the detection of the mecA gene from methicillin-resistant Staphylococcus aureus (MRSA) cultures. The assay uses a mecA probe (DNA-RNA-DNA) labeled with fluorescein at the 5' terminus and biotin at the 3' terminus. The CPT reaction occurs at a constant temperature, which allows the probe to anneal to the target DNA. RNase H cuts the RNA portion of the probe, allowing the cleaved fragments to dissociate from the target DNA, making the target available for further cycling. The strip detection step uses a nitrocellulose membrane with streptavidin and immunoglobulin G antibody impregnated on the surface. In the absence of the mecA gene, the uncut probe is bound to an antifluorescein-gold conjugate and is then captured by the streptavidin to form a test line. In the presence of the mecA gene, the probe is cut and no test line is formed on the strip. A screen of 324 S. aureus clinical isolates by the CPT-strip assay showed a 99.4% sensitivity and a 100% specificity compared to the results of PCR for the detection of the mecA gene. Specificity testing showed that the CPT-strip assay did not exhibit any cross-reactivity with a panel of mecA-negative non-S. aureus isolates. The CPT-strip assay is simple and does not require sophisticated equipment. Furthermore, the assay takes 1.5 h starting from a primary culture to the time to detection of the mecA gene in S. aureus isolates. | lld:pubmed |
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| pubmed-article:10878037 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10878037 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10878037 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:10878037 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10878037 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:10878037 | pubmed:issn | 0095-1137 | lld:pubmed |
| pubmed-article:10878037 | pubmed:author | pubmed-author:ZhuZZ | lld:pubmed |
| pubmed-article:10878037 | pubmed:author | pubmed-author:FongW KWK | lld:pubmed |
| pubmed-article:10878037 | pubmed:author | pubmed-author:BekkaouiFF | lld:pubmed |
| pubmed-article:10878037 | pubmed:author | pubmed-author:MarostenmakiJ... | lld:pubmed |
| pubmed-article:10878037 | pubmed:author | pubmed-author:ModrusanZZ | lld:pubmed |
| pubmed-article:10878037 | pubmed:author | pubmed-author:McNevinJ PJP | lld:pubmed |
| pubmed-article:10878037 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10878037 | pubmed:volume | 38 | lld:pubmed |
| pubmed-article:10878037 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10878037 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10878037 | pubmed:pagination | 2525-9 | lld:pubmed |
| pubmed-article:10878037 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:10878037 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10878037 | pubmed:articleTitle | Rapid solid-phase immunoassay for detection of methicillin-resistant Staphylococcus aureus using cycling probe technology. | lld:pubmed |
| pubmed-article:10878037 | pubmed:affiliation | ID Biomedical Corp., Bothell, Washington 98011, USA. | lld:pubmed |
| pubmed-article:10878037 | pubmed:publicationType | Journal Article | lld:pubmed |
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