| pubmed-article:10868930 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10868930 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:10868930 | lifeskim:mentions | umls-concept:C0030281 | lld:lifeskim |
| pubmed-article:10868930 | lifeskim:mentions | umls-concept:C0596508 | lld:lifeskim |
| pubmed-article:10868930 | lifeskim:mentions | umls-concept:C1441547 | lld:lifeskim |
| pubmed-article:10868930 | lifeskim:mentions | umls-concept:C1882115 | lld:lifeskim |
| pubmed-article:10868930 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:10868930 | pubmed:dateCreated | 2000-7-6 | lld:pubmed |
| pubmed-article:10868930 | pubmed:abstractText | Embryonic stem (ES) cells display the ability to differentiate in vitro into a variety of cell lineages. Using a cell-trapping system, we have obtained an insulin-secreting cell clone from undifferentiated ES cells. The construction used allows the expression of a neomycin selection system under the control of the regulatory regions of the human insulin gene. The chimeric gene also contained a hygromycin resistance gene (pGK-hygro) to select transfected cells. A resulting clone (IB/3x-99) containing 16.5 ng/microg protein of total insulin displays regulated hormone secretion in vitro in the presence of various secretagogues. Clusters obtained from this clone were implanted (1 x 10(6) cells) in the spleen of streptozotocin-induced diabetic animals. Transplanted animals correct hyperglycemia within 1 week and restore body weight in 4 weeks. Whereas an intraperitoneal glucose tolerance test showed a slower recovery in transplanted versus control mice, blood glucose normalization after a challenge meal was similar. This approach opens new possibilities for tissue transplantation in the treatment of type 1 and type 2 diabetes and offers an alternative to gene therapy. | lld:pubmed |
| pubmed-article:10868930 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10868930 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10868930 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:10868930 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10868930 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10868930 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10868930 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:10868930 | pubmed:issn | 0012-1797 | lld:pubmed |
| pubmed-article:10868930 | pubmed:author | pubmed-author:RocheEE | lld:pubmed |
| pubmed-article:10868930 | pubmed:author | pubmed-author:SmithG HGH | lld:pubmed |
| pubmed-article:10868930 | pubmed:author | pubmed-author:BerrySS | lld:pubmed |
| pubmed-article:10868930 | pubmed:author | pubmed-author:ReidJ JJJ | lld:pubmed |
| pubmed-article:10868930 | pubmed:author | pubmed-author:MartínFF | lld:pubmed |
| pubmed-article:10868930 | pubmed:author | pubmed-author:León-QuintoTT | lld:pubmed |
| pubmed-article:10868930 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10868930 | pubmed:volume | 49 | lld:pubmed |
| pubmed-article:10868930 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10868930 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10868930 | pubmed:pagination | 157-62 | lld:pubmed |
| pubmed-article:10868930 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:10868930 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10868930 | pubmed:articleTitle | Insulin-secreting cells derived from embryonic stem cells normalize glycemia in streptozotocin-induced diabetic mice. | lld:pubmed |
| pubmed-article:10868930 | pubmed:affiliation | Institute of Bioengineering, Universidad Miguel Hernandez, San Juan, Alicante, Spain. bernat.soria@umh.es | lld:pubmed |
| pubmed-article:10868930 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10868930 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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