Linked Life Data

10802169

Source:http://linkedlifedata.com/resource/pubmed/id/10802169

Statements in which the resource exists.
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pubmed-article:10802169pubmed:abstractTextThe gbpC gene encoding the glucan-binding protein C which is involved in dextran (glucan)-dependent aggregation (ddag) of Streptococcus mutans has been identified by random mutagenesis. We analyzed ddag(-) mutants containing the intact gbpC gene and found that these mutants possessed a large and characteristic duplication of a region of the chromosome which was responsible for the phenotype. Based upon characterization of these duplications, we developed a strategy to introduce a duplication into any specific region of the chromosome of these organisms. The 690-bp gene responsible for the ddag(-) phenotype was identified within a 60-kb region by observing ddag (positive or negative) phenotypes of successively constructed specific duplication mutants.lld:pubmed
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pubmed-article:10802169pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:10802169pubmed:articleTitleConstruction of region-specific partial duplication mutants (merodiploid mutants) to identify the regulatory gene for the glucan-binding protein C gene in vivo in Streptococcus mutans.lld:pubmed
pubmed-article:10802169pubmed:affiliationOral Health Science Center, Tokyo Dental College, 2-2, Masago 1-chome, Mihama-ku, Chiba City, Japan. yusato@tdc.ac.jplld:pubmed
pubmed-article:10802169pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:10802169pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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