| pubmed-article:10656669 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10656669 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:10656669 | lifeskim:mentions | umls-concept:C0024264 | lld:lifeskim |
| pubmed-article:10656669 | lifeskim:mentions | umls-concept:C1268930 | lld:lifeskim |
| pubmed-article:10656669 | lifeskim:mentions | umls-concept:C1527148 | lld:lifeskim |
| pubmed-article:10656669 | lifeskim:mentions | umls-concept:C0332583 | lld:lifeskim |
| pubmed-article:10656669 | lifeskim:mentions | umls-concept:C0185125 | lld:lifeskim |
| pubmed-article:10656669 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:10656669 | lifeskim:mentions | umls-concept:C0303920 | lld:lifeskim |
| pubmed-article:10656669 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:10656669 | pubmed:dateCreated | 2000-2-10 | lld:pubmed |
| pubmed-article:10656669 | pubmed:abstractText | Green fluorescent protein (GFP) transgenic (GFP+) mice express GFP in most tissues except erythrocytes and hair. Immune responses of GFP+ mouse and their application to studies of lymphocyte development were investigated. Flow cytometric analyses revealed that differentiation patterns of lymphocytes from GFP+ mice are equivalent to those from parental C57BL/6 mice. There was no difference in mature T-cell proliferative ability in response to allogeneic stimulator cells or anti-CD3epsilon stimulation between GFP+ and C57BL/6 mice. Furthermore, the anti-OVA antibody response of GFP+ mice was also the same as that of C57BL/6 mice. Taken together, these results show no immunological differences between GFP+ and C57BL/6 mice. Bone marrow transplantation and in vitro thymus reconstitution experiments were performed in an attempt to apply the GFP+ mice to the analysis of lymphocyte development. When bone marrow cells from GFP+ mice were transplanted. T and B lymphocytes containing GFP developed normally in scid recipients. Next we examined intrathymic T-cell development by hanging drop culture methods. GFP+ and CD4+8+ immature T-cells developed normally from bone marrow cells in the reconstituted thymus. The experimental system using hematopoietic cells from GFP+ mice is a powerful tool for visualizing lymphocyte development. | lld:pubmed |
| pubmed-article:10656669 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10656669 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10656669 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10656669 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10656669 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10656669 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10656669 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10656669 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10656669 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10656669 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:10656669 | pubmed:issn | 0165-2478 | lld:pubmed |
| pubmed-article:10656669 | pubmed:author | pubmed-author:OkabeMM | lld:pubmed |
| pubmed-article:10656669 | pubmed:author | pubmed-author:YamamotoHH | lld:pubmed |
| pubmed-article:10656669 | pubmed:author | pubmed-author:KohamaYY | lld:pubmed |
| pubmed-article:10656669 | pubmed:author | pubmed-author:IwaoMM | lld:pubmed |
| pubmed-article:10656669 | pubmed:author | pubmed-author:IkawaMM | lld:pubmed |
| pubmed-article:10656669 | pubmed:author | pubmed-author:TsujikawaKK | lld:pubmed |
| pubmed-article:10656669 | pubmed:author | pubmed-author:KawakamiNN | lld:pubmed |
| pubmed-article:10656669 | pubmed:author | pubmed-author:NishizawaFF | lld:pubmed |
| pubmed-article:10656669 | pubmed:author | pubmed-author:SakaneNN | lld:pubmed |
| pubmed-article:10656669 | pubmed:author | pubmed-author:FukadaS ISI | lld:pubmed |
| pubmed-article:10656669 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10656669 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:10656669 | pubmed:volume | 70 | lld:pubmed |
| pubmed-article:10656669 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10656669 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10656669 | pubmed:pagination | 165-71 | lld:pubmed |
| pubmed-article:10656669 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:10656669 | pubmed:meshHeading | pubmed-meshheading:10656669... | lld:pubmed |
| pubmed-article:10656669 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10656669 | pubmed:articleTitle | Green fluorescent protein-transgenic mice: immune functions and their application to studies of lymphocyte development. | lld:pubmed |
| pubmed-article:10656669 | pubmed:affiliation | Department of Immunology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Japan. | lld:pubmed |
| pubmed-article:10656669 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10656669 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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