| pubmed-article:10627036 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10627036 | lifeskim:mentions | umls-concept:C0009063 | lld:lifeskim |
| pubmed-article:10627036 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:10627036 | lifeskim:mentions | umls-concept:C0699857 | lld:lifeskim |
| pubmed-article:10627036 | lifeskim:mentions | umls-concept:C0301630 | lld:lifeskim |
| pubmed-article:10627036 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:10627036 | lifeskim:mentions | umls-concept:C1314939 | lld:lifeskim |
| pubmed-article:10627036 | pubmed:dateCreated | 2000-2-17 | lld:pubmed |
| pubmed-article:10627036 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10627036 | pubmed:abstractText | We have conducted the genetic analysis of fermentative nitrate reduction in Clostridium perfringens, a strict anaerobic bacterium. Nitrate reductase (NarA) was purified from the cytoplasmic fraction of the organism. Using a degenerate primer designed from its N-terminal amino acid sequence, a 9.5 kb fragment containing seven ORFs was cloned. The molecular mass and N-terminal amino acid sequence predicted from the nucleotide sequence of ORF 4 coincided with those determined for the purified NarA, indicating that ORF 4 corresponds to a narA gene. ORFs 5 and 6 encode a 15.4 kDa ferredoxin-like protein containing four iron-sulfur clusters and a 45 kDa protein homologous to NADH oxidase, respectively. Analyses involving primer extension and Northern blotting revealed that these three ORFs are transcribed as a polycistronic message. The ORF 5- and ORF 6-encoded proteins were shown by immunoblotting to be synthesized by cells grown in the presence of nitrate. Thus, these two proteins are likely to function as electron-transfer components in nitrate reduction in C perfringens. The 9.5 kb fragment and a downstream region of 6.1 kb do not contain any genes involved in nitrate uptake or nitrite reduction. Instead, all 5 ORFs downstream of ORF 6 are homologous to genes reported for molybdopterin biosynthesis, unlike the genomic organization already determined for the respiratory and assimilatory nitrate-reduction systems. The evolutionary relationships between these two nitrate-reduction systems and the fermentative one based on the results of comparative genetic analysis are discussed. | lld:pubmed |
| pubmed-article:10627036 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10627036 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10627036 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10627036 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10627036 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10627036 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10627036 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10627036 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10627036 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10627036 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10627036 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10627036 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10627036 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10627036 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10627036 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10627036 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10627036 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:10627036 | pubmed:issn | 1350-0872 | lld:pubmed |
| pubmed-article:10627036 | pubmed:author | pubmed-author:KatayamaSS | lld:pubmed |
| pubmed-article:10627036 | pubmed:author | pubmed-author:TaniguchiYY | lld:pubmed |
| pubmed-article:10627036 | pubmed:author | pubmed-author:FujinagaKK | lld:pubmed |
| pubmed-article:10627036 | pubmed:author | pubmed-author:OkabeAA | lld:pubmed |
| pubmed-article:10627036 | pubmed:author | pubmed-author:SunYY | lld:pubmed |
| pubmed-article:10627036 | pubmed:author | pubmed-author:MinamiJJ | lld:pubmed |
| pubmed-article:10627036 | pubmed:author | pubmed-author:MatsushitaOO | lld:pubmed |
| pubmed-article:10627036 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10627036 | pubmed:volume | 145 ( Pt 12) | lld:pubmed |
| pubmed-article:10627036 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10627036 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10627036 | pubmed:pagination | 3377-87 | lld:pubmed |
| pubmed-article:10627036 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:10627036 | pubmed:meshHeading | pubmed-meshheading:10627036... | lld:pubmed |
| pubmed-article:10627036 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10627036 | pubmed:articleTitle | Analysis of genes involved in nitrate reduction in Clostridium perfringens. | lld:pubmed |
| pubmed-article:10627036 | pubmed:affiliation | Department of Microbiology, Faculty of Medicine, Kagawa Medical University, Kita-gun, Japan. | lld:pubmed |
| pubmed-article:10627036 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10627036 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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