| pubmed-article:10592099 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10592099 | lifeskim:mentions | umls-concept:C0006142 | lld:lifeskim |
| pubmed-article:10592099 | lifeskim:mentions | umls-concept:C0007082 | lld:lifeskim |
| pubmed-article:10592099 | lifeskim:mentions | umls-concept:C0005953 | lld:lifeskim |
| pubmed-article:10592099 | lifeskim:mentions | umls-concept:C0229664 | lld:lifeskim |
| pubmed-article:10592099 | lifeskim:mentions | umls-concept:C0035379 | lld:lifeskim |
| pubmed-article:10592099 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:10592099 | lifeskim:mentions | umls-concept:C0032520 | lld:lifeskim |
| pubmed-article:10592099 | lifeskim:mentions | umls-concept:C0220825 | lld:lifeskim |
| pubmed-article:10592099 | lifeskim:mentions | umls-concept:C0205221 | lld:lifeskim |
| pubmed-article:10592099 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:10592099 | pubmed:dateCreated | 1999-12-28 | lld:pubmed |
| pubmed-article:10592099 | pubmed:abstractText | This study evaluated the specificity and sensitivity of the reverse transcriptase/polymerase chain reaction (RT-PCR) for carcinoembryonic antigen (CEA) for identification of breast tumour cells in bone marrow and in peripheral blood. Using one primer set from the A2/B3 domains of CEA with five to seven mismatches to other CEA-family members allowed reproducible detection of 1 colon tumour cell and 10 breast tumour cells in 10(7) mononuclear cells. Bone marrow samples from 181 patients with breast cancer were analysed by CEA-RT-PCR; 50 of these samples were analysed in parallel by routine immunocytochemistry. CEA-mRNA-positive bone marrow cells were found in 27.6% of the patients (50/181) with breast cancer. Five immunocytochemistry-positive samples were negative when analysed by CEA-RT-PCR. Limiting factors in the detection of micrometastatic breast tumour cells by CEA-RT-PCR are the heterogeneity of the tumour cells and the deficient expression of CEA in some of these cells. However, CEA-RT-PCR using the specific primer could detect 1 colon tumour cell in 1 x 10(7) normal peripheral blood mononuclear cells. | lld:pubmed |
| pubmed-article:10592099 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10592099 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10592099 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10592099 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10592099 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10592099 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10592099 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:10592099 | pubmed:issn | 0171-5216 | lld:pubmed |
| pubmed-article:10592099 | pubmed:author | pubmed-author:ThompsonJJ | lld:pubmed |
| pubmed-article:10592099 | pubmed:author | pubmed-author:KaulSS | lld:pubmed |
| pubmed-article:10592099 | pubmed:author | pubmed-author:BastertGG | lld:pubmed |
| pubmed-article:10592099 | pubmed:author | pubmed-author:EichlerAA | lld:pubmed |
| pubmed-article:10592099 | pubmed:author | pubmed-author:ZhongX YXY | lld:pubmed |
| pubmed-article:10592099 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10592099 | pubmed:volume | 125 | lld:pubmed |
| pubmed-article:10592099 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10592099 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10592099 | pubmed:pagination | 669-74 | lld:pubmed |
| pubmed-article:10592099 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:10592099 | pubmed:meshHeading | pubmed-meshheading:10592099... | lld:pubmed |
| pubmed-article:10592099 | pubmed:meshHeading | pubmed-meshheading:10592099... | lld:pubmed |
| pubmed-article:10592099 | pubmed:meshHeading | pubmed-meshheading:10592099... | lld:pubmed |
| pubmed-article:10592099 | pubmed:meshHeading | pubmed-meshheading:10592099... | lld:pubmed |
| pubmed-article:10592099 | pubmed:meshHeading | pubmed-meshheading:10592099... | lld:pubmed |
| pubmed-article:10592099 | pubmed:meshHeading | pubmed-meshheading:10592099... | lld:pubmed |
| pubmed-article:10592099 | pubmed:meshHeading | pubmed-meshheading:10592099... | lld:pubmed |
| pubmed-article:10592099 | pubmed:meshHeading | pubmed-meshheading:10592099... | lld:pubmed |
| pubmed-article:10592099 | pubmed:meshHeading | pubmed-meshheading:10592099... | lld:pubmed |
| pubmed-article:10592099 | pubmed:meshHeading | pubmed-meshheading:10592099... | lld:pubmed |
| pubmed-article:10592099 | pubmed:meshHeading | pubmed-meshheading:10592099... | lld:pubmed |
| pubmed-article:10592099 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10592099 | pubmed:articleTitle | Evaluation of the reverse transcriptase/polymerase chain reaction for carcinoembryonic antigen for the detection of breast cancer dissemination in bone marrow and peripheral blood. | lld:pubmed |
| pubmed-article:10592099 | pubmed:affiliation | Laboratory of Oncology, Department of Obstetrics and Gynaecology, University Hospital, Heidelberg, Germany. Zhong@ubaclu.unibas.ch | lld:pubmed |
| pubmed-article:10592099 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10592099 | lld:pubmed |
