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pubmed-article:10581434pubmed:abstractTextStoichiometric relationships for biological reactions involving intermediate formation are developed from microbial reaction fundamentals and thermodynamic principles. Biological reactions proceed through intermediates, which sequester carbon and electrons whenever their degradation is relatively slow. Modeling intermediate formation and subsequent utilization requires evaluation of the distribution of electrons, energy, and macronutrients (C and N) between energy-generating pathways and cell-synthesis pathways for each step in the mineralization of the primary electron-donor substrate. We describe how energy and electron balances are utilized to predict the stoichiometry for each step of a multi-step degradation process. Each stoichiometric relationship developed predicts substrate utilization, cell growth, and the formation of other products (e.g., H(2)CO(3) or H(+)) for one step in the pathway to full mineralization. A modeling example demonstrates how different kinetics for each step in the degradation of nitrilotriacetic acid (NTA) leads to observed patterns in experimental results, such as a delay in the release of H(2)CO(3) after NTA is removed from solution.lld:pubmed
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pubmed-article:10581434pubmed:issn0006-3592lld:pubmed
pubmed-article:10581434pubmed:authorpubmed-author:RittmannB EBElld:pubmed
pubmed-article:10581434pubmed:authorpubmed-author:VanBriesenJ...lld:pubmed
pubmed-article:10581434pubmed:copyrightInfoCopyright 2000 John Wiley & Sons, Inc.lld:pubmed
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pubmed-article:10581434pubmed:authorsCompleteYlld:pubmed
pubmed-article:10581434pubmed:pagination35-52lld:pubmed
pubmed-article:10581434pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:10581434pubmed:year2000lld:pubmed
pubmed-article:10581434pubmed:articleTitleMathematical description of microbiological reactions involving intermediates.lld:pubmed
pubmed-article:10581434pubmed:affiliationDepartment of Civil Engineering, Northwestern University, Evanston, IL, USA. jeanne@cmu.edulld:pubmed
pubmed-article:10581434pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:10581434pubmed:publicationTypeResearch Support, U.S. Gov't, Non-P.H.S.lld:pubmed