| pubmed-article:10570141 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10570141 | lifeskim:mentions | umls-concept:C1882071 | lld:lifeskim |
| pubmed-article:10570141 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:10570141 | lifeskim:mentions | umls-concept:C0332621 | lld:lifeskim |
| pubmed-article:10570141 | lifeskim:mentions | umls-concept:C0205360 | lld:lifeskim |
| pubmed-article:10570141 | lifeskim:mentions | umls-concept:C1705294 | lld:lifeskim |
| pubmed-article:10570141 | lifeskim:mentions | umls-concept:C0441712 | lld:lifeskim |
| pubmed-article:10570141 | pubmed:issue | 24 | lld:pubmed |
| pubmed-article:10570141 | pubmed:dateCreated | 2000-1-6 | lld:pubmed |
| pubmed-article:10570141 | pubmed:abstractText | A major activity of molecular chaperones is to prevent aggregation and refold misfolded proteins. However, when allowed to form, protein aggregates are refolded poorly by most chaperones. We show here that the sequential action of two Escherichia coli chaperone systems, ClpB and DnaK-DnaJ-GrpE, can efficiently solubilize excess amounts of protein aggregates and refold them into active proteins. Measurements of aggregate turbidity, Congo red, and 4,4'-dianilino-1, 1'-binaphthyl-5,5'-disulfonic acid binding, and of the disaggregation/refolding kinetics by using a specific ClpB inhibitor, suggest a mechanism where (i) ClpB directly binds protein aggregates, ATP induces structural changes in ClpB, which (ii) increase hydrophobic exposure of the aggregates and (iii) allow DnaK-DnaJ-GrpE to bind and mediate dissociation and refolding of solubilized polypeptides into native proteins. This efficient mechanism, whereby chaperones can catalytically solubilize and refold a wide variety of large and stable protein aggregates, is a major addition to the molecular arsenal of the cell to cope with protein damage induced by stress or pathological states. | lld:pubmed |
| pubmed-article:10570141 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10570141 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10570141 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10570141 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10570141 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:10570141 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:10570141 | pubmed:author | pubmed-author:TomoyasuTT | lld:pubmed |
| pubmed-article:10570141 | pubmed:author | pubmed-author:BukauBB | lld:pubmed |
| pubmed-article:10570141 | pubmed:author | pubmed-author:GoloubinoffPP | lld:pubmed |
| pubmed-article:10570141 | pubmed:author | pubmed-author:MogkAA | lld:pubmed |
| pubmed-article:10570141 | pubmed:author | pubmed-author:ZviA PAP | lld:pubmed |
| pubmed-article:10570141 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10570141 | pubmed:day | 23 | lld:pubmed |
| pubmed-article:10570141 | pubmed:volume | 96 | lld:pubmed |
| pubmed-article:10570141 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10570141 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10570141 | pubmed:pagination | 13732-7 | lld:pubmed |
| pubmed-article:10570141 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:10570141 | pubmed:meshHeading | pubmed-meshheading:10570141... | lld:pubmed |
| pubmed-article:10570141 | pubmed:meshHeading | pubmed-meshheading:10570141... | lld:pubmed |
| pubmed-article:10570141 | pubmed:meshHeading | pubmed-meshheading:10570141... | lld:pubmed |
| pubmed-article:10570141 | pubmed:meshHeading | pubmed-meshheading:10570141... | lld:pubmed |
| pubmed-article:10570141 | pubmed:meshHeading | pubmed-meshheading:10570141... | lld:pubmed |
| pubmed-article:10570141 | pubmed:meshHeading | pubmed-meshheading:10570141... | lld:pubmed |
| pubmed-article:10570141 | pubmed:meshHeading | pubmed-meshheading:10570141... | lld:pubmed |
| pubmed-article:10570141 | pubmed:meshHeading | pubmed-meshheading:10570141... | lld:pubmed |
| pubmed-article:10570141 | pubmed:meshHeading | pubmed-meshheading:10570141... | lld:pubmed |
| pubmed-article:10570141 | pubmed:meshHeading | pubmed-meshheading:10570141... | lld:pubmed |
| pubmed-article:10570141 | pubmed:meshHeading | pubmed-meshheading:10570141... | lld:pubmed |
| pubmed-article:10570141 | pubmed:meshHeading | pubmed-meshheading:10570141... | lld:pubmed |
| pubmed-article:10570141 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10570141 | pubmed:articleTitle | Sequential mechanism of solubilization and refolding of stable protein aggregates by a bichaperone network. | lld:pubmed |
| pubmed-article:10570141 | pubmed:affiliation | Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel. pierre@vms.huji.ac.il | lld:pubmed |
| pubmed-article:10570141 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10570141 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:944750 | entrezgene:pubmed | pubmed-article:10570141 | lld:entrezgene |
| entrez-gene:947077 | entrezgene:pubmed | pubmed-article:10570141 | lld:entrezgene |
| entrez-gene:944753 | entrezgene:pubmed | pubmed-article:10570141 | lld:entrezgene |
| entrez-gene:947097 | entrezgene:pubmed | pubmed-article:10570141 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10570141 | lld:pubmed |
