Linked Life Data

1055383

Source:http://linkedlifedata.com/resource/pubmed/id/1055383

Statements in which the resource exists.
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pubmed-article:1055383pubmed:abstractTextPseudomonas phaseolicola infected with bacteriophage phi 6 synthesized all three viral double-stranded RNA segments, three single-stranded RNAs, and three replicative intermediate-like RNAs in the presence of rifampin. The single-stranded RNA intermediates sedimented and electrophoresed along with melted viral double-stranded RNA, annealed with melted viral double-stranded RNA, and were transient in nature. The relative amounts of the single-stranded RNA intermediates varied during the infection cycle and were altered in the presence of chloramphenicol. The replicative intermediate-like RNAs sedimented faster than double-stranded RNA, failed to enter 2.5% polyacrylamide gels, eluted with double-stranded RNA from a CF-11 cellulose column, were precipitated with single-stranded RNA in 2 M LiC1, and yielded three genome-size pieces of double-stranded RNA upon digestion with RNase. These results are consistent with the hypothesis that complementary strands of the phi 6 double-stranded RNAs are synthesized asynchronously during the infection cycle.lld:pubmed
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pubmed-article:1055383pubmed:authorpubmed-author:VidaverA KAKlld:pubmed
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pubmed-article:1055383pubmed:dateRevised2010-9-3lld:pubmed
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pubmed-article:1055383pubmed:articleTitleIntermediates in the biosynthesis of double-stranded ribonucleic acids of bacteriophage phi 6.lld:pubmed
pubmed-article:1055383pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:1055383pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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