pubmed-article:10550470 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:10550470 | lifeskim:mentions | umls-concept:C1040568 | lld:lifeskim |
pubmed-article:10550470 | lifeskim:mentions | umls-concept:C0699900 | lld:lifeskim |
pubmed-article:10550470 | lifeskim:mentions | umls-concept:C0243125 | lld:lifeskim |
pubmed-article:10550470 | lifeskim:mentions | umls-concept:C1526874 | lld:lifeskim |
pubmed-article:10550470 | lifeskim:mentions | umls-concept:C1522492 | lld:lifeskim |
pubmed-article:10550470 | lifeskim:mentions | umls-concept:C1548602 | lld:lifeskim |
pubmed-article:10550470 | pubmed:issue | 5 | lld:pubmed |
pubmed-article:10550470 | pubmed:dateCreated | 1999-12-29 | lld:pubmed |
pubmed-article:10550470 | pubmed:abstractText | The anaerobic bacterium Desulfobacterium cetonicum oxidized m-cresol completely with sulfate as electron acceptor. During growth, 3-hydroxybenzylsuccinate (identified by gas chromatography/mass spectroscopy and by comparison of high-performance liquid chromatography retention time and UV spectrum with a chemically synthesized reference compound) accumulated in the medium. This finding indicates that the methyl group of m-cresol is activated by addition to fumarate as in the case of anaerobic toluene metabolism. In cell-free extracts of D. cetonicum, the formation of 3-hydroxybenzylsuccinate from m-cresol and fumarate was detected at an activity of 0.5 nmol min(-1) (mg protein)(-1). This reaction depended strictly on anoxic assay conditions. Treatment with air resulted in a complete loss of activity; however, some activity could be recovered after restoring anoxic conditions. The activity was slightly membrane-associated. 3-Hydroxybenzylsuccinate was degraded via CoA thioesterification and further oxidation to 3-hydroxybenzoyl-CoA as subsequent steps in the degradation pathway. | lld:pubmed |
pubmed-article:10550470 | pubmed:language | eng | lld:pubmed |
pubmed-article:10550470 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:10550470 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:10550470 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:10550470 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:10550470 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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pubmed-article:10550470 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:10550470 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:10550470 | pubmed:month | Nov | lld:pubmed |
pubmed-article:10550470 | pubmed:issn | 0302-8933 | lld:pubmed |
pubmed-article:10550470 | pubmed:author | pubmed-author:SchinkBB | lld:pubmed |
pubmed-article:10550470 | pubmed:author | pubmed-author:MüllerJ AJA | lld:pubmed |
pubmed-article:10550470 | pubmed:author | pubmed-author:GalushkoA SAS | lld:pubmed |
pubmed-article:10550470 | pubmed:author | pubmed-author:KapplerAA | lld:pubmed |
pubmed-article:10550470 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:10550470 | pubmed:volume | 172 | lld:pubmed |
pubmed-article:10550470 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:10550470 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:10550470 | pubmed:pagination | 287-94 | lld:pubmed |
pubmed-article:10550470 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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pubmed-article:10550470 | pubmed:meshHeading | pubmed-meshheading:10550470... | lld:pubmed |
pubmed-article:10550470 | pubmed:year | 1999 | lld:pubmed |
pubmed-article:10550470 | pubmed:articleTitle | Anaerobic degradation of m-cresol by Desulfobacterium cetonicum is initiated by formation of 3-hydroxybenzylsuccinate. | lld:pubmed |
pubmed-article:10550470 | pubmed:affiliation | Fakultät für Biologie, Universität Konstanz, Fach M654, D-78457 Konstanz, Germany. jochen_mueller@hotmail.com | lld:pubmed |
pubmed-article:10550470 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:10550470 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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